Skip to main content
SpringerLink
Log in

Development of a flow-injection analysis (FIA) enzyme sensor for fructosyl amine monitoring

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

An enzyme-sensor system with flow-injection analysis (FIA) has been developed for the detection of fructosyl amine compounds; the sensor utilizes fructosyl amine oxidase isolated from the marine yeast Pichia sp. N1-1 strain. With this FIA system 0.2 to 10 mmol L–1 fructosyl valine can be determined. The sensor is approximately five times more sensitive to fructosyl valine, a model compound for glycated hemoglobin HbAff1c, than to N ε-fructosyl lysine, a model compound for glycated albumin. This FIA system can also be used to detect fructosyl dipeptides. The operational stability of the sensor enabled more than 120 consecutive sample injections over a period of approximately 20 h.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan, Japan

    Kinuko Ogawa, Fumimasa Ishimura, Wakako Tsugawa, Stefano Ferri & Koji Sode

  2. Analytical Biochemistry, University of Potsdam, Karl-Liebknecht-Strasse 24–25, 14476 Golm, Germany, Germany

    Daniela Stöllner, Frieder Scheller & Axel Warsinke

Authors
  1. Kinuko Ogawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daniela Stöllner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Frieder Scheller
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Axel Warsinke
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fumimasa Ishimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wakako Tsugawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Stefano Ferri
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Koji Sode
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ogawa, K., Stöllner, D., Scheller, F. et al. Development of a flow-injection analysis (FIA) enzyme sensor for fructosyl amine monitoring. Anal Bioanal Chem 373, 211–214 (2002). https://doi.org/10.1007/s00216-002-1319-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-002-1319-6

Search

Navigation