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Ventral tegmental area α6β2 nicotinic acetylcholine receptors modulate phasic dopamine release in the nucleus accumbens core

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Abstract

Rationale

Phasic dopamine (DA) signaling underlies reward learning. Cholinergic and glutamatergic inputs into the ventral tegmental area (VTA) are crucial for modulating burst firing activity and subsequent phasic DA release in the nucleus accumbens (NAc), but the specific VTA nicotinic receptor subtypes that regulate phasic DA release have not been identified.

Objective

The goal was to determine the role of VTA N-methyl-d-aspartate receptors (NMDARs) and specific subtypes of nicotinic acetylcholine receptors (nAChRs) in regulating phasic DA release in the NAc core.

Methods

Fast-scan cyclic voltammetry in anesthetized rats was combined with intra-VTA micro-infusion to evaluate the ability of glutamatergic and cholinergic drugs to modulate stimulated phasic DA release in the NAc core.

Results

VTA NMDAR blockade with AP-5 decreased, while VTA NMDAR activation with NMDA increased NAc peak phasic DA release. Intra-VTA administration of the nonspecific nAChR antagonist mecamylamine produced a persistent decrease in phasic DA release. Infusion of the α6-selective antagonist α-conotoxin MII (α-ctx MII) produced a robust, but transient decrease in phasic DA, whereas infusion of selective doses of either the α4β2-selective antagonist, dihydro-beta-erythroidine, or the α7 antagonist, methyllycaconitine, had no effect. Co-infusion of AP-5 and α-ctx MII produced a similar phasic DA decrease as either drug alone, with no additive effect.

Conclusions

The results suggest that VTA α6β2 nAChRs, but not α4β2 or α7 nAChRs, regulate phasic DA release in the NAc core and that VTA α6β2 nAChRs and NMDA receptors act at a common site or target to regulate NAc phasic DA signaling.

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Acknowledgments

This work was supported by NIH GM103801 and GM48677 (JMM) and by a NSF Graduate Research Fellowship (RJW).

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Authors and Affiliations

  1. Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, CT, 06511, USA

    Robert Wickham & Nii A. Addy

  2. Department of Psychiatry, Yale University School of Medicine, 34 Park Street, 3rd Floor Research, New Haven, CT, 06520, USA

    Wojciech Solecki, Liza Rathbun & Nii A. Addy

  3. Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, 06511, USA

    Nii A. Addy

  4. Department of Biology, University of Utah, Salt Lake City, UT, 84112, USA

    J. Michael McIntosh

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  1. Robert Wickham
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  2. Wojciech Solecki
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  5. Nii A. Addy
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Correspondence to Nii A. Addy.

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Wickham, R., Solecki, W., Rathbun, L. et al. Ventral tegmental area α6β2 nicotinic acetylcholine receptors modulate phasic dopamine release in the nucleus accumbens core. Psychopharmacology 229, 73–82 (2013). https://doi.org/10.1007/s00213-013-3082-0

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  • DOI: https://doi.org/10.1007/s00213-013-3082-0

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