Abstract
Rationale
Activation of the immune system typically occurs on a subchronic or chronic basis (e.g., in response to bacterial or viral insults). However, analyses of the effects of cytokine treatments have typically involved acute treatments, and limited data are available concerning the behavioral and central neurochemical impact of subchronic interleukin-1β (IL-1β) administration.
Objectives
Several peripheral and central effects of IL-1β treatment were assessed following single or repeated bolus injections or after infusion of the cytokine (through Alzet minipumps) over several days.
Results
The impact of an acute bolus injection of IL-1β (1.0 μg) on plasma corticosterone and on circulating IL-1β, IL-6, and TNF-α were diminished following 5-day IL-1β treatment, although high levels of sickness were still apparent. When IL-1β (1.0 or 2.0 μg/day) was continuously infused over 3 days, plasma corticosterone and sickness were elevated, but these effects were attenuated after 7 days (subchronic) of treatment. As well, the effects of IL-1β treatment on diurnal variations of motor activity diminished over days. Despite the diminution of the behavioral and neuroendocrine effects of the cytokine after treatment 7 days, subchronic IL-1β infusion altered prefrontal cortical and hippocampal serotonin and norepinephrine utilization, and within these regions, the messenger RNA (mRNA) expression of IL-1β, IL-6, TNF-α, and their receptors, as well as that of 5-HT2C, 5-HT1B receptors, and p11, was increased.
Discussion
The findings indicate that peripheral cytokine infusion markedly influences central cytokine mRNA expression and also influences 5-HT turnover, which might contribute to behavioral changes elicited by IL-1β.
Similar content being viewed by others
References
Anisman H, Merali Z (1999) Anhedonic and anxiogenic effects of cytokine exposure. Adv Exp Med Biol 461:199–233
Anisman H, Poulter MO, Gandhi R, Merali Z, Hayley S (2007) Interferon-alpha effects are exaggerated when administered on a psychosocial stressor backdrop: cytokine, corticosterone and brain monoamine variations. J Neuroimmunol 86:45–53
Anisman H, Du L, Palkovits M, Faludi G, Kovacs GG, Szontagh-Kishazi P, Merali Z, Poulter MO (2008) Serotonin receptor subtype and p11 mRNA expression in stress-relevant brain regions of suicide and control subject. J Psychiatry Neurosci 33:131–141
Bocci V (1991) Interleukins: clinical pharmacokinetics and practical implications. Clin Pharmacokin 21:274–284
Bonaccorso S, Maier SF, Meltzer HY, Maes M (2003) Behavioral changes in rats after acute, chronic and repeated administration of interleukin-1beta: relevance for affective disorders. J Affect Disord 77:143–148
Brebner K, Hayley S, Merali Z, Anisman H (2000) Synergistic effects of interleukin-1, interleukin-6, and TNF-α: neuroendocrine, neurochemical and behavioral changes. Neuropsychopharmacology 22:566–580
Brummett BH, Boyle SH, Siegler IC, Kuhn CM, Ashley-Koch A, Jonassaint CR, Züchner S, Collins A, Williams RB (2007) Effects of environmental stress and gender on associations among symptoms of depression and the serotonin transporter gene linked polymorphic region (5-HTTLPR). Behav Genet 38:34–43
Busbridge NJ, Dascombe MJ, Rothwell NJ (1993) Chronic effects of interleukin-1β on fever, oxygen consumption and food intake in the rat. Horm Metab Res 25:222–227
Chen J, Sochivko D, Beck H, Marechal D, Wiestler OD, Becker AJ (2001) Activity-induced expression of common reference genes in individual cns neurons. Lab Invest 81:913–916
Dantzer R (2001) Cytokine-induced sickness behavior: mechanisms and implications. Ann N Y Acad Sci 933:222–234
Day HE, Curran EJ, Watson SJ Jr., Akil H (1999) Distinct neurochemical populations in the rat central nucleus of the amygdala and bed nucleus of the stria terminalis: evidence for their selective activation by interleukin-1beta. J Comp Neurol 413:113–128
Finck BN, Johnson RW (1997) Anorexia, weight loss and increased plasma interleukin-6 caused by chronic intracerebroventricular infusion of interleukin-1beta in the rat. Brain Res 761:333–337
Franklin KBJ, Paxinos G (1997) A stereotaxic atlas of the mouse brain. Academic Press, San Diego, CA
Frasure-Smith N, Lesperance F (2005) Reflections on depression as a cardiac risk factor. Psychosom Med 67:S19–25
Gandhi R, Hayley S, Gibb J, Merali Z, Anisman H (2007) Influence of poly I:C on sickness behaviors, plasma cytokines, corticosterone and central monoamine activity: moderation by social stressors. Brain Behav Immun 21:477–489
Griffin WS, Liu L, Li Y, Mrak RE, Barger SW (2006) Interleukin-1 mediates Alzheimer and Lewy body pathologies. J Neuroinflammation 3:5
Hayley S, Brebner K, Lacosta S, Merali Z, Anisman H (1999) Sensitization to the effects of tumor necrosis factor-a: neuroendocrine, central monoamine, and behavioral variations. J Neurosci 19:5654–5665
Hill AG, Jacobson L, Gonzalez J, Rounds J, Majzoub JA, Wilmore DW (1996) Chronic central nervous system exposure to interleukin-1 beta causes catabolism in the rat. Am J Physiol 271:R1142–1148, Nov
Hillhouse EW, Kida S, Iannotti F (1998) Middle cerebral artery occlusion in the rat causes a biphasic production of immunoreactive interleukin-1beta in the cerebral cortex. Neurosci Lett 249:177–179
Johnson AB, Bake S, Lewis DK, Sohrabji F (2006) Temporal expression of IL-1beta protein and mRNA in the brain after systemic LPS injection is affected by age and estrogen. J Neuroimmunol 174:82–91
Konsman JP, Parnet P, Dantzer R (2002) Cytokine-induced sickness behavior: mechanisms and implications. Trends Neurosci 25:154–159
Kubera M, Lin AH, Kenis G, Bosmans E, van Bockstaele D, Maes M (2001) Anti-Inflammatory effects of antidepressants through suppression of the interferon-gamma/interleukin-10 production ratio. J Clin Psychopharmaco 21:199–206
Ling PR, Schwartz JH, Jeevanandam M, Gauldie J, Bistrian BR (1996) Metabolic changes in rats during a continuous infusion of recombinant interleukin-1. Am J Physiol 270:E305–312
Linthorst ACE, Flachskamm C, Müller-Preuss P, Holsboer F, Reul JMHM (1995) Effect of bacterial endotoxin and interleukin-1β on hippocampal serotonergic neurotransmission, behavioral activity, and free corticosterone levels: An in vivo microdialysis study. J Neurosci 15:2920–2934
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 4:402–408
Maes M (1999) Major depression and activation of the inflammatory response system. Adv Exp Med Biol 461:25–46
McDonald WM, Richard IH, DeLong MR (2003) Prevalence, etiology, and treatment of depression in Parkinson’s disease. Biol Psychiatry 54:363–375
Merali Z, Du L, Hrdina P, Palkovits M, Faludi G, Poulter MO, Anisman H (2004) Dysregulation in the suicide brain: mRNA expression of corticotropin releasing hormone receptors and GABAA receptor subunits in frontal cortical brain region. J Neurosci 24:1478–1485
MohanKumar SM, Smith CL, MohanKumar PS (2003) Central adaptation to chronic administration of interleukin-1beta (IL-1beta) in rats. Brain Res Bull 62:71–76
Mrosovsky N, Molony LA, Conn CA, Kluger MJ (1989) Anorexic effects of interleukin-1 in the rat. Am J Physiol 257:R1315–R1321
Nestler EJ, Barrot M, DiLeone RJ, Eisch AJ, Gold SJ, Monteggia LM (2002) Neurobiology of depression. Neuron 34:13–25
Nutt DJ (2002) The neuropharmacology of serotonin and noradrenaline in depression. Int Clin Psychopharmacol 17:S1–S12
Nuyen J, Schellevis FG, Satariano WA, Spreeuwenberg PM, Birkner MD, van den Bos GA, Groenewegen PP (2006) Comorbidity was associated with neurologic and psychiatric diseases: a general practice-based controlled study. J Clin Epidemiol 59:1274–1284
Raison CL, Capuron L, Miller AH (2006) Cytokines sing the blues: inflammation and the pathogenesis of depression. Trends Immunol 27:24–31
Rivest S, Lacroix S, Vallieres L, Nadeau S, Zhang J, Laflamme N (2000) How the blood talks to the brain parenchyma and the paraventricular nucleus of the hypothalamus during systemic inflammatory and infectious stimuli. Proc Soc Exp Biol Med 223:22–38
Ruzek MC, Miller AH, Opal SM, Pearce BD, Biron CA (1997) Characterization of early cytokine responses and an interleukin (IL)-6-dependent pathway of endogenous glucocorticoid induction during murine cytomegalovirus infection. J Exp Med 185(7):1185–1192, Apr 7
Sawada M, Imamura K, Nagatsu T (2006) Role of cytokines in inflammatory process in Parkinson’s disease. J Neural Transm 70:373–381
Schmidt HD, Duman RS (2007) The role of neurotrophic factors in adult hippocampal neurogenesis, antidepressant treatments and animal models of depressive-like behavior. Behav Pharmacol 18:391–418
Schmidt ED, Janszen AW, Wouterlood FG, Tilders FJ (1995) Interleukin-1-induced long-lasting changes in hypothalamic corticotropin-releasing hormone (CRH)-neurons and hyperresponsiveness of the hypothalamus–pituitary–adrenal axis. J Neurosci 15:7417–7426
Silverman MN, Miller AH, Biron CA, Pearce BD (2004) Characterization of an interleukin-6- and adrenocorticotropin-dependent, immune-to-adrenal pathway during viral infection. Endocrinology. 145(8):3580–3589, Aug
Stockmeier CA (2003) Involvement of serotonin in depression: evidence from postmortem and imaging studies of serotonin receptors and the serotonin transporter. J Psychiatr Res 37:357–373
Svenningsson P, Chergui K, Rachleff I, Flajolet M, Zhang X, El Yacoubi M et al (2006) Alterations in 5-HT1B receptor function by p11 in depression-like states. Science 311:77–80
Teeling JL, Felton LM, Deacon RM, Cunningham C, Rawlins JN, Perry VH (2007) Sub-pyrogenic systemic inflammation impacts on brain and behavior, independent of cytokines. Brain Behav Immun 21:836–850
Tilders FJ, Schmidt ED (1999) Cross-sensitization between immune and non-immune stressors. A role in the etiology of depression? Adv Exp Med Biol 461:179–197
Tong L, Balazs R, Soiampornkul R, Thangnipon W, Cotman CW (2007) Interleukin-1beta impairs brain derived neurotrophic factor-induced signal transduction. Neurobiol Aging (in press)
Turrin NP, Gayle D, Ilyin SE, Flynn MC, Langhans W, Schwartz GJ, Plata-Salaman CR (2001) Pro-inflammatory and anti-inflammatory cytokine mRNA induction in the periphery and brain following intraperitoneal administration of bacterial lipopolysaccharide. Brain Res Bull 54:443–453
van der Meer MJ, Lebeau A, Duval P, Grimaldi B, Terlain B, Kerdelhue BAR (1995) Synergism between IL-1 beta and TNF-alpha on the activity of the pituitary–adrenal axis and on food intake of rats. Am J Physiol 268:E551–E557
van Haasteren GA, van der Meer MJ, Hermus AR, Linkels E, Klootwijk W, Kaptein E, van Toor H, Sweep CG, Visser TJ, de Greef WJ (1994) Different effects of continuous infusion of interleukin-1 and interleukin-6 on the hypothalamic–hypophysial–thyroid axis. Endocrinology 135:1336–1345
Wang MJ, Kuo JS, Lee WW, Huang HY, Chen WF, Lin SZ (2006) Translational event mediates differential production of tumor necrosis factor-alpha in hyaluronan-stimulated microglia and macrophages. J Neurochem 97:857–871
Wilhelm K, Siegel JE, Finch AW, Hadzi-Pavlovic D, Mitchell PB, Parker G, Schofield PR (2007) The long and the short of it: associations between 5-HTT genotypes and coping with stress. Psychosom Med 69:614–620
Yoshimura A, Naka T, Kubo M (2007) SOCS proteins, cytokine signalling and immune regulation. Nat Rev Immunol 7:454–465
Zhu Y, Saito K, Murakami Y, Asano M, Iwakura Y, Seishima M (2006) Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia. Neurosci Lett 393:122–126
Author information
Authors and Affiliations
Corresponding author
Additional information
The research was supported by the Canadian Institutes of Health Research (CIHR) and by the Canada Foundation for Innovation (CFI). Hymie Anisman and Shawn Hayley hold Canada Research Chairs in Behavioral Neuroscience and in Neuroscience, respectively. None of the authors have a financial interest in either CIHR or CFI.
Rights and permissions
About this article
Cite this article
Anisman, H., Gibb, J. & Hayley, S. Influence of continuous infusion of interleukin-1β on depression-related processes in mice: corticosterone, circulating cytokines, brain monoamines, and cytokine mRNA expression. Psychopharmacology 199, 231–244 (2008). https://doi.org/10.1007/s00213-008-1166-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00213-008-1166-z