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D2 but not D1 dopamine receptor stimulation augments brain signaling involving arachidonic acid in unanesthetized rats

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Abstract

Rationale and objectives

Signal transduction involving the activation of phospholipase A2 (PLA2) to release arachidonic acid (AA) from membrane phospholipids, when coupled to dopamine D1- and D2-type receptors, can be imaged in rats having a chronic unilateral lesion of the substantia nigra. It is not known, however, if the signaling responses occur in the absence of a lesion. To determine this, we used our in vivo fatty acid method to measure signaling in response to D1 and D2 receptor agonists given acutely to unanesthetized rats.

Methods

[1-14C]AA was injected intravenously in unanesthetized rats, and incorporation coefficients k* for AA (brain radioactivity/integrated plasma radioactivity) were measured using quantitative autoradiography in 61 brain regions. The animals were administered i.v. the D2 receptor agonist, quinpirole (1 mg kg−1, i.v.), the D1 receptor agonist SKF-38393 (5 mg kg−1, i.v.), or vehicle/saline.

Results

Quinpirole increased k* significantly in multiple brain regions rich in D2-type receptors, whereas SKF-38393 did not change k* significantly in any of the 61 regions examined.

Conclusions

In the intact rat brain, D2 but not D1 receptors are coupled to the activation of PLA2 and the release of AA.

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Abbreviations

rCMRglc :

regional cerebral metabolic rate for glucose

PLA2 :

phospholipase A2

AA:

arachidonic acid

PLC:

phospholipase C

sn :

stereospecifically numbered

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Authors and Affiliations

  1. Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bldg. 9, Room 1S128, Bethesda, MD, 20892, USA

    Abesh Kumar Bhattacharjee, Lisa Chang, Ho-Joo Lee, Richard P. Bazinet, Ruth Seemann & Stanley I. Rapoport

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  1. Abesh Kumar Bhattacharjee
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  2. Lisa Chang
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  3. Ho-Joo Lee
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  4. Richard P. Bazinet
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  6. Stanley I. Rapoport
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Correspondence to Abesh Kumar Bhattacharjee.

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Bhattacharjee, A.K., Chang, L., Lee, HJ. et al. D2 but not D1 dopamine receptor stimulation augments brain signaling involving arachidonic acid in unanesthetized rats. Psychopharmacology 180, 735–742 (2005). https://doi.org/10.1007/s00213-005-2208-4

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  • DOI: https://doi.org/10.1007/s00213-005-2208-4

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