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Utilization of plant-derived Myricetin molecule coupled with ultrasound for the synthesis of gold nanoparticles against breast cancer

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Abstract

Phytochemical mediated synthesis of nanoparticles has gained great interest in the field of cancer therapeutics. We attempted a simple and stable synthesis of gold nanoparticles (AuNPs) with Myricetin (Myr) adopting ultrasound-assisted method. Further, we evaluated anticancer activity of the synthesized nanoparticles. The physico-chemical properties of biosynthesized Myr-AuNPs were characterized by UV-visible spectrophotometer, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and particle size analysis. The study reports of Myr-AuNPs showed spherical-shaped particles with a size of < 50 nm. Stability of the particles was increased in various physiological media. Furthermore, the graph theoretical network analysis of Myr-AuNPs indicated that the probable binding with the mTOR is an effective target for breast cancer cells. In silico molecular docking study of Myr-AuNPs in human mTOR kinase was found to be strong binding. The IC50 value of Myr-AuNPs was calculated as 13 μg mL−1 against MCF-7 cell line. The AO/EB and DAPI stainings confirmed the anticancer activity by Myr-AuNPs-treated cells showed a good proportion of dead cells evidenced with formation of pro-apoptotic bodies. In addition, Myr-AuNPs exhibited depolarization of mitochondrial membrane potential and production of reactive oxygen species. This study proves that Myr-AuNPs holds great promise to use against breast cancer as a potent anticancer drug.

A schematic representation for the biosynthesis of Myr-AuNPs.

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Acknowledgments

The authors are grateful to the Management of Kalasalingam University, Krishnankoil, India, for research fellowships and utilizing research facilities. We thank Mr. P. Kathirvel, Mr. V. Krishnaprabhu Technicians for FTIR and XRD analysis, Sir CV Raman-KS Krishnan International Research Center, Kalasalingam University, Krishnankoil, India, and SAIF, STIC, Cochin University, Cochin, Kerala, India, for TEM analysis. We also thank Prof. Z. Maciej Gliwicz, Ms. Ewa Babkiewicz, and Dr. Piotr Maszczyk, Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warsaw, Poland, for timely help and support.

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Authors and Affiliations

  1. Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, Tamil Nadu, 626126, India

    Uma Priya Mohan, Sankarganesh Devaraj, Adhvitha Premanand, Sankarganesh Arunachalam & Selvaraj Kunjiappan

  2. School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, Tamil Nadu, 600048, India

    Bhaskaran Sriram & Davoodbasha MubarakAli

  3. Department of Pharmaceutical Chemistry, Saraswathi College of Pharmacy, NH-24, Anwarpur, Pilkhuwa, Hapur, Uttar Pradesh, 245304, India

    Theivendren Panneerselvam

  4. Division of Bioengineering, Incheon National University, Incheon, Republic of Korea

    Davoodbasha MubarakAli

  5. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, M S R Nagar, Bengaluru, Karnataka, 560054, India

    Pavadai Parasuraman

  6. School of Mechanical Engineering, Vellore Institute of Technology, Vellore, Tamilnadu, 632014, India

    Ponnusamy Palanisamy

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  1. Uma Priya Mohan
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Contributions

UPM, SB, AP, PP, and SK conceived and designed the research. UPM, SB, AP, PP, PP, DMA, SK, and TP conducted the experiments. KS, SA, SD, and DMA analyzed the data. KS, DMA, and TP wrote the manuscript. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Sankarganesh Arunachalam or Selvaraj Kunjiappan.

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All procedures were performed in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and were approved by the local animal protection committee.

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No animals/humans were used for studies that are the basis of this research.

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The authors declare that there are no conflicts of interests.

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Mohan, U.P., Sriram, B., Panneerselvam, T. et al. Utilization of plant-derived Myricetin molecule coupled with ultrasound for the synthesis of gold nanoparticles against breast cancer. Naunyn-Schmiedeberg's Arch Pharmacol 393, 1963–1976 (2020). https://doi.org/10.1007/s00210-020-01874-6

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