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Mangiferin ameliorates the intestinal inflammatory response and the impaired gastrointestinal motility in mouse model of postoperative ileus

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Abstract

Our previous study has shown that mangiferin (MGF), a glucosylxanthone from Mangifera indica, exerts gastrointestinal prokinetic action involving a cholinergic mechanism. Postoperative ileus (POI) is a temporary disturbance in gastrointestinal motility following surgery, and intestinal inflammatory response plays a critical role in the pathogenesis of POI. The present study investigated to know whether MGF having anti-inflammatory and prokinetic actions can ameliorate the intestinal inflammation and impaired gastrointestinal transit seen in the mouse model of POI. Experimental POI was induced in adult male Swiss mice by standardized small intestinal manipulation (IM). Twenty-four hours later, gastrointestinal transit was assessed by charcoal transport. MGF was administered orally 1 h before the measurement of GIT. To evaluate the inflammatory response, plasma levels of proinflammatory cytokines TNF-α, IL-1β, IL-6, and chemokine MCP-1, and the myeloperoxidase activity, nitrate/nitrite level, and histological changes of ileum were determined in mice treated or not with MGF. Experimental POI in mice was characterized by decreased gastrointestinal transit and marked intestinal and systemic inflammatory response. MGF treatment led to recovery of the delayed intestinal transit induced by IM. MGF in ileum significantly inhibited the myeloperoxidase activity, a marker of neutrophil infiltration, and nitrate/nitrite level and reduced the plasma levels of TNF-α, IL-1β, IL-6, and MCP-1 as well. MGF treatment ameliorates the intestinal inflammatory response and the impaired gastrointestinal motility in the mouse model of POI.

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Acknowledgments

This research was supported by the grants and fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP). We are grateful to Aguinéa Rocha de Morais for their excellent technical assistance. We are also grateful to Prof. Geanne Matos de Andrade, Kelly Rose Tavares Neves, and Francisco Arnaldo Viana Lima for immunohistochemical analysis.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Faculty of Medicine, INCT–IBISAB–Brazilian Semi-Arid Institute of Biomedicine, 60430-270, Fortaleza, Ceará, Brazil

    Talita Cavalcante Morais, Bruno Rodrigues Arruda, Vietla Satyanarayana Rao & Flavia Almeida Santos

  2. Department of Organic and Inorganic Chemistry, Federal University of Ceará, 60451-970, Fortaleza, Ceará, Brazil

    Hebert de Sousa Magalhães & Maria Teresa Salles Trevisan

  3. Laboratory of Pathology and Legal Medicine, Faculty of Veterinary, State University of Ceará, 60740-000, Fortaleza, Ceará, Brazil

    Daniel de Araújo Viana

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  1. Talita Cavalcante Morais
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  2. Bruno Rodrigues Arruda
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  7. Flavia Almeida Santos
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Correspondence to Flavia Almeida Santos.

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Morais, T.C., Arruda, B.R., de Sousa Magalhães, H. et al. Mangiferin ameliorates the intestinal inflammatory response and the impaired gastrointestinal motility in mouse model of postoperative ileus. Naunyn-Schmiedeberg's Arch Pharmacol 388, 531–538 (2015). https://doi.org/10.1007/s00210-015-1095-4

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