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Differential amino acid transmission in the locus coeruleus of Wistar Kyoto and spontaneously hypertensive rats

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Abstract

In addition to differences in their blood pressure, Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) are known to differ in their emotional behaviour. The neurochemistry underlying these differences is not well understood. In the present study the release rates of the two main regulatory amino acids in the locus coeruleus, glutamate and gamma-aminobutyric acid (GABA), were monitored in WKY rats and SHR to investigate whether basal and/or challenged neurotransmission differs between these strains. The strains differed in their basal blood pressure (WKY 102±2 mmHg, SHR 140±4 mmHg), as well as in their emotional behaviour, since WKY rats displayed enhanced anxiety-related behaviour in the open field test (time in centre: WKY 197±40 s/30 min, SHR 741±93 s/30 min). Basal glutamate and GABA release rates did not differ between WKY rats and SHR. A rise in blood pressure induced by intravenous infusion of noradrenaline for 10 min enhanced GABA release in WKY rats by 60%, while no effect was observed in SHR. Glutamate release did not respond to experimental hypertension in both strains. Intravenous infusion of sodium nitroprusside led to a fall in blood pressure, which was less pronounced and was of shorter duration in WKY rats than in SHR. The depressor response had no effect on amino acid release in the locus coeruleus of both strains. Mild stress induced by noise or tail pinch led to slight rises in arterial blood pressure (10 mmHg and 20 mmHg respectively), which were similar in WKY rats and SHR. Tail pinch enhanced the release rates of glutamate and GABA in the locus coeruleus of WKY rats and SHR; however, no strain differences were noted. Noise stress did not significantly influence amino acid release. These findings demonstrate that SHR and WKY rats differ in GABAergic neurotransmission, which is revealed in response to specific cardiovascular challenges, but not to mild stressors. The observed lack of GABA response to blood pressure elevation in SHR may reflect a disturbed mechanism counteracting high blood pressure, possibly contributing to hypertension in this strain.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Innsbruck, Peter-Mayr-Strasse 1, 6020, Innsbruck, Austria

    S. T. Kaehler, P. Salchner, N. Singewald & A. Philippu

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  1. S. T. Kaehler
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  2. P. Salchner
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  3. N. Singewald
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  4. A. Philippu
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Correspondence to S. T. Kaehler.

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Kaehler, S.T., Salchner, P., Singewald, N. et al. Differential amino acid transmission in the locus coeruleus of Wistar Kyoto and spontaneously hypertensive rats. Naunyn-Schmiedeberg's Arch Pharmacol 370, 381–387 (2004). https://doi.org/10.1007/s00210-004-0987-5

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  • DOI: https://doi.org/10.1007/s00210-004-0987-5

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