Skip to main content
SpringerLink
Log in

Purification and properties of an extremely thermostable NADP+-specific glutamate dehydrogenase from Archaeoglobus fulgidus

  • Short communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

NADP+-specific glutamate dehydrogenase (EC 1.4.1.4) was purified to homogeneity from the extremely thermophilic, strictly anaerobic, sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324. The native enzyme (263 kDa) is composed of subunits of mol. mass 46 kDa, suggesting a hexameric structure. The temperature optimum for enzyme activity was > 95° C. The enzyme was highly thermostable, having a half-life of 140 min at 100° C. Potassium phosphate, KCl, and NaCl enhanced the thermal stability and increased the rate of activity three- to fourfold. The N-terminal 26-amino-acid sequence showed a high degree of similarity to glutamate dehydrogenases from Pyrococcus spp. and Thermococcus spp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Bergen, Jahnebakken 5, N-5020 Bergen, Norway Tel.: +47-55-582657; Fax +47-55-589671 e-mail: nils.birkeland@im.uib.no, , , , , , NO

    Nina Aalén, Ida Helene Steen, Nils-Kåre Birkeland & Torleiv Lien

Authors
  1. Nina Aalén
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ida Helene Steen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nils-Kåre Birkeland
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Torleiv Lien
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 25 March 1997 / Accepted: 11 July 1997

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aalén, N., Steen, I., Birkeland, NK. et al. Purification and properties of an extremely thermostable NADP+-specific glutamate dehydrogenase from Archaeoglobus fulgidus. Arch Microbiol 168, 536–539 (1997). https://doi.org/10.1007/s002030050533

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002030050533

Search

Navigation