Abstract
During the investigation, soil actinomycetes were isolated from Kathlekanu swamp forest and the crude ethyl acetate extract from the potent isolate KF15 was analysed with GC–MS and HPTLC to identify bioactive metabolites. The crude extract was examined for in-vitro antifungal activity on pathogens of chilli; MTT cytotoxicity assay was performed against HeLa cell line to determine the anticancer potential. The isolate Streptomyces sp. strain KF15 exhibited antagonistic activity against fungal pathogens by inhibiting growth and altering growth pattern with increased antimicrobial activity in dose-dependent manner. GC–MS revealed many bioactive compounds and HPTLC depicted metabolite fingerprint. The IC50 of 99.85 µg/ml indicated the high potential of KF15 extract to prevent proliferation of HeLa cells. Therefore, the findings of this study indicate that the crude extract from Streptomyces sp. strain KF15 contains antifungal and anticancer metabolites; further study on purification could help in controlling many fungal diseases as well as cervical cancer in humans.
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Acknowledgements
The authors are thankful to P.G. Department of Studies in Botany for extending laboratory facility and to Sophisticated Analytical Instrumentation Facility (SAIF), University Scientific Instrumentation Centre, Karnatak University, Dharwad, for providing necessary instrumentation facilities. The authors are thankful to Department of Plant pathology, U.A.S., Dharwad, for providing fungal pathogens of chilli.
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The research concept was designed by SN and MPB with supervision done by SN. MPB carried out the experimental methodologies and SN and RSK did the data validation. MPB wrote the original draft of the manuscript along with review and editing. All the authors have read and agreed to the published version of the manuscript.
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Bhat, M.P., Nayaka, S. & Kumar, R.S. A swamp forest Streptomyces sp. strain KF15 with broad spectrum antifungal activity against chilli pathogens exhibits anticancer activity on HeLa cells. Arch Microbiol 204, 540 (2022). https://doi.org/10.1007/s00203-022-03147-7
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DOI: https://doi.org/10.1007/s00203-022-03147-7