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Biogenesis of selenium nanospheres using Halomonas venusta strain GUSDM4 exhibiting potent environmental applications

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Abstract

Selenite reducing bacterial strain (GUSDM4) isolated from Mandovi estuary of Goa, India was identified as Halomonas venusta based on 16S rRNA gene sequence analysis. Its maximum tolerance level for sodium selenite (Na2SeO3) was 100 mM. The 2, 3-diaminonaphthalene-based spectroscopic analysis demonstrated 96 and 93% reduction of 2 and 4 mM Na2SeO3 respectively to elemental selenium (Se0) during the late stationary growth phase. Biosynthesis of Se nanoparticles (SeNPs) commenced within 4 h during the log phase, which was evident from the brick red color in the growth medium and a characteristic peak at 265 nm revealed by UV–Vis spectrophotometry. The intracellular periplasmic synthesis of SeNPs in GUSDM4 was confirmed by transmission electron microscopy (TEM). Characterization of SeNPs by X-ray crystallography, TEM and energy-dispersive X-ray analysis (EDAX) clearly demonstrated spherical SeNPs of 20–80 nm diameter with hexagonal crystal lattice. SeNPs (0.8 and 1 mg/L) primed seeds under arsenate [As(V)] stress showed increase in shoot length, root length and biomass by 1.4-, 1.5- and 1.1-fold respectively, as compared to As(V) primed seeds alone. The proline and phenolic content in seeds primed with SeNPs under arsenate stress showed alleviated levels proving its ameliorative potential. SeNPs also demonstrated anti-biofilm activity at 20 µg/mL against human pathogens which was evident by scanning electron microscopic (SEM) analysis. SeNPs interestingly revealed mosquito larvicidal activity also. Therefore, these studies have clearly demonstrated amazing potential of the marine bacterium, Halomonas venusta in biosynthesis of SeNPs and their applications as ameliorative, anti-biofilm and mosquito larvicidal agents which is the first report of its kind.

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Availability of data and material

The datasets generated during and/or analyzed during the current study are available in the GenBank repository (MG430411). All data generated or analyzed during this study are included in this published article (and its supplementary information files).

Code availability

All the software used in the current study has been mentioned in the article with appropriate reference.

References

  • Ábrahám E, Hourton-Cabassa C, Erdei L, Szabados L (2010) Methods for determination of proline inplants. In: Plant stress tolerance. Humana Press, pp 317–331

  • Akçay FA, Avcı A (2020) Effects of process conditions and yeast extract on the synthesis of selenium nanoparticles by a novel indigenous isolate Bacillus sp. EKT1 and characterization of nanoparticles. Arch Microbiol 202(8):2233–2243

    Article  PubMed  CAS  Google Scholar 

  • Allan CB, Lacourciere GM, Stadtman TC (1999) Responsiveness of selenoproteins to dietary selenium. Ann Rev Nutr 19:1–16

    Article  CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1999) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  Google Scholar 

  • Arthur JR, McKenzie RC, Beckett GJ (2003) Selenium in the immune system. J Nutr 133:1457S-1459S

    Article  CAS  PubMed  Google Scholar 

  • Cartes P, Gianfreda L, Paredes C, Mora ML (2011) Selenium uptake and its antioxidant role in ryegrass cultivars as affected by selenite seed pelletization. J Soil Sci Plant Nutr 11(4):1–14

    Article  Google Scholar 

  • Chen JS (2012) An original discovery: selenium deficiency and Keshan disease (an endemic heart disease). Asia Pac J Clin Nutr 21:320–326

    PubMed  Google Scholar 

  • Dhanjal S, Cameotra SS (2010) Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil. Microb Cell Fact 9(1):1–11

    Article  CAS  Google Scholar 

  • Ellis RH, Roberts EH (1981) The quantification of ageing and survival in orthodox seeds. Seed Sci Technol (Netherlands)

  • Eswayah AS, Smith TJ, Gardiner PH (2016) Microbial transformations of selenium species of relevance to bioremediation. Appl Environ Microbiol 82(16):4848–4859

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Forootanfar H, Adeli-Sardou M, Nikkhoo M, Mehrabani M, Amir-Heidari B, Shahverdi AR, Shakibaie M (2014) Antioxidant and cytotoxic effect of biologically synthesized selenium nanoparticles in comparison to selenium dioxide. J Trace Elem Med Bio 28:75–79

    Article  CAS  Google Scholar 

  • Ghosh A, Mohod AM, Paknikar KM, Jain RK (2008) Isolation and characterization of selenite-and selenate-tolerant microorganisms from selenium-contaminated sites. World J Microbiol Biotechnol 24:1607–1611

    Article  CAS  Google Scholar 

  • Ghosh A, Chowdhury N, Chandra G (2012) Plant extracts as potential mosquito larvicides. IJMR 135:581

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hunter WJ, Manter DK (2009) Reduction of selenite to elemental red selenium by Pseudomonas sp. strain CA5. Curr Microbiol 58:493–498

    Article  CAS  PubMed  Google Scholar 

  • Javed S, Sarwar A, Tassawar M, Faisal M (2015) Conversion of selenite to elemental selenium by indigenous bacteria isolated from polluted areas. Chem Spec Bioavailab 7(4):162–168

    Article  CAS  Google Scholar 

  • Kessi J, Ramuz M, Wehrli E, Spycher M, Bachofen R (1999) Reduction of selenite and detoxification of elemental selenium by the Phototrophic Bacterium Rhodospirillum rubrum. Appl Environ Microbiol 65(11):4734–4740

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khaliq A, Ali S, Hameed A, Farooq MA, Farid M, Shakoor MB, Rizwan M (2016) Silicon alleviates nickel toxicity in cotton seedlings through enhancing growth, photosynthesis, and suppressing Ni uptake and oxidative stress. Arch Agron Soil Sci 62(5):633–647

    Article  CAS  Google Scholar 

  • Khanolkar DS, Dubey SK, Naik MM (2015) Biotransformation of tributyltin chloride to less toxic dibutyltin dichloridee and monobutyltin trichloride by Klebsiella pneumoniae strain SD9. Int Biodeter Biodegr 104:212–218

    Article  CAS  Google Scholar 

  • Klaus T, Joerger R, Olsson E, Granqvist C (1999) Silver-based crystalline nanoparticles, microbially fabricated. PNAS (0) 96(24):13611–13614

    Article  CAS  Google Scholar 

  • Mishra RR, Prajapati S, Das J, Dangar TK, Das N, Thatoi H (2011) Reduction of selenite to red elemental selenium by moderately halotolerant Bacillus megaterium strains isolated from Bhitarkanika mangrove soil and characterization of reduced product. Chemosphere 84:231–1237

    Article  CAS  Google Scholar 

  • Morris J, Crane S (2013) Selenium toxicity from a misformulated dietary supplement, adverse health effects, and the temporal response in the nail biologic monitor. Nutrients 5:1024–1057

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Moulick D, Ghosh D, Santra SC (2016) Evaluation of effectiveness of seed priming with selenium in rice during germination under arsenic stress. Plant Physiol Biochem 109:571–578

    Article  CAS  PubMed  Google Scholar 

  • Mujawar SY, Shamim K, Vaigankar DC, Dubey SK (2019) Arsenite biotransformation and bioaccumulation by Klebsiella pneumoniae strain SSSW7 possessing arsenite oxidase (aioA) gene. Biometals 32:65–76

    Article  CAS  PubMed  Google Scholar 

  • Naik MM, Dubey SK (2013) Lead resistant bacteria: lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotoxicol Environ Saf 98:1–7

    Article  CAS  PubMed  Google Scholar 

  • Naik MM, Dubey SK (2017) Marine pollution and microbial remediation. Springer, Singapore

    Book  Google Scholar 

  • Narasingarao P, Häggblom MM (2007) Identification of anaerobic selenate-respiring bacteria from aquatic sediments. Appl Environ Microbiol 73:3519–3527

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Oremland RS, Herbel MJ, Blum JS, Langley S, Beveridge TJ, Ajayan PM, Sutto T, Ellis AV, Curran S (2004) Structural and spectral features of selenium nanospheres produced by Se-respiring bacteria. Appl Environ Microbiol 70:52–60

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ouédraogo O, Chételat J, Amyot M (2015) Bioaccumulation and trophic transfer of mercury and selenium in African sub-tropical fluvial reservoirs food webs (Burkina Faso). PLoS ONE 10(4):0123048

    Article  CAS  Google Scholar 

  • Ranjard L, Prigent-Combaret C, Nazaret S, Cournoyer B (2002) Methylation of inorganic and organic selenium by the bacterial thiopurine methyltransferase. J Bacteriol 184:3146–3149

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rathgeber C, Yurkova N, Stackebrandt E, Beatty JT, Yurkov V (2002) Isolation of tellurite-and selenite-resistant bacteria from hydrothermal vents of the juan de fuca ridge in the Pacific ocean. Appl Environ Microbiol 68:4613–4622

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rauschenbach I, Narasingarao P, Häggblom MM (2011) Desulfurispirillum indicum sp. nov, a selenate-and selenite-respiring bacterium isolated from an estuarine canal. Int J Syst Evol Microbiol 61:654–658

    Article  CAS  PubMed  Google Scholar 

  • Samant S, Naik M, Parulekar K, Charya L, Vaigankar D (2018) Selenium reducing Citrobacter fruendii strain KP6 from Mandovi estuary and its potential application in selenium nanoparticle synthesis. Proc Natl Sci India Sect B: Bio Sci 88:747–754

    Article  CAS  Google Scholar 

  • Shakibaie M, Forootanfar H, Golkari Y, Mohammadi-Khorsand T, Shakibaie MR (2015) Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis. J Trace Elem Med Biol 29:235–241

    Article  CAS  PubMed  Google Scholar 

  • Shirsat S, Kadam A, Naushad M, Mane RS (2015) Selenium nanostructures: microbial synthesis and applications. RSC Adv 5(112):92799–92811

    Article  CAS  Google Scholar 

  • Siddique T, Zhang Y, Okeke BC, Frankenberger WT Jr (2006) Characterization of sediment bacteria involved in selenium reduction. Bioresour Technol 97:1041–1049

    Article  CAS  PubMed  Google Scholar 

  • Soda S, Takahashi H, Kagami T, Miyake M, Notaguchi E, Sei K, Iwasaki N, Ike M (2012) Biotreatment of selenium refinery wastewater using pilot-scale granular sludge and swim-bed bioreactors augmented with a selenium-reducing bacterium Pseudomonas stutzeri NT-I. J Water Treat Biol 48(2):63–71

    Article  Google Scholar 

  • Sowndarya P, Ramkumar G, Shivakumar MS (2017) Green synthesis of selenium nanoparticles conjugated Clausena dentata plant leaf extract and their insecticidal potential against mosquito vectors. Artif Cell Nanomed B 451:490–1495

    Google Scholar 

  • Srivastava P, Kowshik M (2016) Anti-neoplastic selenium nanoparticles from Idiomarina sp. PR58-8. Enzyme Microb Technol 95:192–200

    Article  CAS  PubMed  Google Scholar 

  • Srivastava N, Mukhopadhyay M (2013) Biosynthesis and structural characterization of selenium nanoparticles mediated by Zooglearamigera. Powder Technol 244:26–29

    Article  CAS  Google Scholar 

  • Srivastava P, Braganca JM, Kowshik M (2014) In vivo synthesis of selenium nanoparticles by Halococcus salifodinae BK18 and their anti-proliferative properties against HeLa cell line. Biotechnol Prog 30:1480–1487

    Article  CAS  PubMed  Google Scholar 

  • Stoeva S, Klabunde KJ, Sorensen CM, Dragieva I (2002) Gram-Scale Synthesis of Monodisperse Gold Colloids by the Solvated Metal Atom Dispersion Method and Digestive Ripening and Their Organization into Two-and Three-Dimensional structures. J Am ChemSoc 124:2305–2311

    Article  CAS  Google Scholar 

  • Sunitha MSL, Prashanth S, Kishor PK (2015) Characterization of arsenic-resistant bacteria and their arsgenotype for metal bioremediation. Int J Sci Eng Res 6:304–309

    Google Scholar 

  • Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica. I.—The quantitativeanalysis of phenolic constituents. J Sci Food Agric 10(1):63–68

    Article  CAS  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tan Y, Yao R, Wang R, Wang D, Wang G, Zheng S (2016) Reduction of selenite to Se (0) nanoparticles by filamentous bacterium Streptomyces sp. ES2–5 isolated from a selenium mining soil. Microb Cell Fact 15:157

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Vaigankar DC, Dubey SK, Mujawar SY, D’Costa A, Shyama SK (2018) Tellurite biotransformation and detoxification by Shewanella baltica with simultaneous synthesis of tellurium nanorods exhibiting photo-catalytic and anti-biofilm activity. Ecotox Environ Safe 165:516–526

    Article  CAS  Google Scholar 

  • Watkinson JH (1966) Fluorimetric determination of selenium in bio-logical material with 2, 3-diaminonaphtalene. Anal Chem 38:92–97

    Article  CAS  PubMed  Google Scholar 

  • Xu XY, McGrath SP, Meharg AA, Zhao FJ (2008) Growing rice aerobically markedly decreases arsenic accumulation. Environ Sci Technol 42(15):5574–5579

    Article  CAS  PubMed  Google Scholar 

  • Yang YC, Lee SG, Lee HK, Kim MK, Lee SH, Lee HS (2002) A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J Agric Food Chem 50:3765–3767

    Article  CAS  PubMed  Google Scholar 

  • Zeng H, Combs G Jr (2008) Selenium as an anticancer nutrient: roles in cell proliferation and tumor cell invasion. J Nutr Biochem 19:1–7

    Article  PubMed  CAS  Google Scholar 

  • Zhao FJ, Ma JF, Meharg AA, McGrath SP (2009) Arsenic uptake and metabolism in plants. New Phytol 181(4):777–794

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors thank Mr Areef Sardar and Mr Girish Prabhu from CSIR-National Institute of Oceanography, Goa for EDAX and XRD analysis respectively. The authors also acknowledge AIIMS, New Delhi for TEM analysis.

Funding

This work was supported by University Grants Commission, New Delhi as a junior research fellow [Ref. no. F/2017–18(SA-III)].

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Authors and Affiliations

  1. Laboratory of Bacterial Genetics and Environmental Biotechnology, Department of Microbiology, Goa University, Taleigao Plateau, Goa, 403206, India

    Diviya Chandrakant Vaigankar, Sajiya Yusuf Mujawar & Santosh Kumar Dubey

  2. Field Unit, ICMR-National Institute of Malarial Research, DHS Building, Campal, Panaji, Goa, 403206, India

    Ajeet Kumar Mohanty

  3. Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India

    Santosh Kumar Dubey

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  1. Diviya Chandrakant Vaigankar
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Contributions

DCV has designed, performed and analyzed the experiments. She has also prepared the draft of the manuscript. SKD professor and mentor has contributed to experimental designs, verified the data, and critically corrected the manuscript to bring in the final form. SYM has assisted in experimental work and making draft manuscript. AKM has helped in designing and conducting mosquito larvicidal assays.

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Correspondence to Santosh Kumar Dubey.

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Conflict of interest

DCV received the grants from University Grants Commission, New Delhi as a junior research fellow [Ref. no. F/2017–18(SA-III)].

SYM declares no conflict of interest. AKM declares no conflict of interest. SKD declares no conflict of interest.

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Communicated by Erko Stackebrandt.

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Vaigankar, D.C., Mujawar, S.Y., Mohanty, A.K. et al. Biogenesis of selenium nanospheres using Halomonas venusta strain GUSDM4 exhibiting potent environmental applications. Arch Microbiol 204, 372 (2022). https://doi.org/10.1007/s00203-022-02977-9

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