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Microbacterium sulfonylureivorans sp. nov., isolated from sulfonylurea herbicides degrading consortium

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Abstract

A novel Gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7116T was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil from Xinjiang. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain LAM7116T was closely related to the members of the genus Microbacterium, with the highest similarity to Microbacterium flavescens DSM 20643T (98.48%) and Microbacterium kitamiense Kitami C2T (98.48%). Strain LAM7116T formed a distinct subclade with M. flavescens DSM 20643T within the genus Microbacterium in the 16S rRNA gene phylogenetic trees. The genomic DNA G + C content of LAM7116T was 69.9 mol%. The digital DNA–DNA hybridization (dDDH) value between strain LAM7116T and M. flavescens DSM 20643T was 27.20%. The average nucleotide identity (ANI) value was 83.96% by comparing the draft genome sequences of strain LAM7116T and M. flavescens DSM 20643T. The major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C17:0, and iso-C16:0. The respiratory menaquinones of strain LAM7116T were MK-13 and MK-14. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified lipid, and an unidentified glycolipid. The peptidoglycan contained the amino acids glycine, lysine, alanine, and glutamic acid. Based on the phenotypic characteristics and genotypic analyses, we consider that strain LAM7116T represents a novel species, for which the name Microbacterium sulfonylureivorans sp. nov. was proposed. The type strain is LAM7116T (= CGMCC 1.16620T = JCM 32823T). Strain LAM7116T secreted auxin IAA and grew well in Ashby nitrogen-free culture medium. Genomic results showed that strain LAM7116T carried the nitrogenase iron protein (nifU and nifR3) gene, which indicated that strain LAM7116T has the potential to fix nitrogen and promote plant growth. At same time, strain LAM7116T can degrade nicosulfuron (a kind of sulfonylurea herbicides) using glucose as carbon source. Microbacterium sulfonylureivorans sp. nov. LAM7116T is a potential candidate for the biofertilizers of organic agriculture areas, and may possess potential to be used in bioremediation of nicosulfuron-contaminated environments.

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Funding

This research was supported by the National Natural Science Foundation of China (NSFC 32070004, 31670006). This work was also supported by Central Public interest Scientific Institution Basal Research Fund (No. Y2021GH18).

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Author notes

  1. Qingyun Ma, Delong Kong, and Qi Zhang have contributed equally to this work.

Authors and Affiliations

  1. Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

    Qingyun Ma, Delong Kong, Xiaoyan Han, Yiqing Zhou, Xu Jiang & Zhiyong Ruan

  2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Qingyun Ma

  3. College of Life Science, Xinjiang Normal University, Ürümqi, 830046, People’s Republic of China

    Juan Che & Wei Zhang

  4. Xinjiang Urumqi Maternal and Child Care Hospital, Ürümqi, 830046, People’s Republic of China

    Qi Zhang

  5. College of Resources and Environment, Tibet Agricultural and Animal Husbandry University, Linzhi, 860000, People’s Republic of China

    Zhiyong Ruan

  6. College of Resources and Environment, China University of Geosciences, Beijing, 10081, People’s Republic of China

    Miaomiao Li

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  1. Qingyun Ma
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Contributions

QYM and DLK: study design; QZ and MML: conceived and supervised the study; DLK: analyzed the data; XYH: prepared the figures; QYM and YQZ: wrote the manuscript; ZYR and WZ: edited the manuscript and reviewed the literature; JC: manuscript revision. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Xu Jiang or Zhiyong Ruan.

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Ma, Q., Kong, D., Zhang, Q. et al. Microbacterium sulfonylureivorans sp. nov., isolated from sulfonylurea herbicides degrading consortium. Arch Microbiol 204, 136 (2022). https://doi.org/10.1007/s00203-021-02750-4

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