Abstract
Genome editing technology has progressed rapidly in recent years. Although traditional gene-editing methods, including homologous recombination, zinc finger endonucleases, and transcription activator-like effector nucleases, have substantial implications for research in genetics and molecular biology, but they have remarkable limitations, including their low efficiency, high error rate, and complex design. A new gene-editing technology, the CRISPR/Cas system, was developed based on studies of archaeal and bacterial immune responses to viruses. Owing to its high target efficiency, simple primer design, and wide applications, the CRISPR/Cas system, whose developers were awarded the Nobel Prize in Chemistry in 2020, has become the dominant genomic editing technology in academia and the pharmaceutical industry. Here, we briefly introduce the CRISPR/Cas system and its main applications for genome engineering, metabolic engineering, and transcriptional regulation in yeast, filamentous fungi, and macrofungi. The polygene and polyploid editing, construction of yeast chromosomes, yeast library creation, regulation of metabolic pathways, and CRISPR activation/CRISPR interference systems are mainly summarized and discussed. The potential applications for the treatment of fungal infections and the further transformation and application of the CRISPR/Cas system in fungi are also proposed and discussed.
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Acknowledgements
This work was supported by National Natural Science Foundation of China grants: 81870778 (BR), 81600858 (BR), 81870759 (LC); Applied Basic Research Programs of Sichuan Province, 2020YJ0227 (BR); the Youth Grant of the Science and Technology Department of Sichuan Province, China, 2017JQ0028 (LC); Innovative Research Team Program of Sichuan Province (LC); Fund of State Key Laboratory of Oral Diseases (SKLOD201913) (BR).
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BL, BR and LC contributed to the conception, writing, interpretation and approval of the manuscript. BL and XC contributed to data collection, interpretation, and final approval of data. XZ, YS, XY, ZZ, YZ and ML contributing the writing, interpretation and approval of the manuscript.
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Liao, B., Chen, X., Zhou, X. et al. Applications of CRISPR/Cas gene-editing technology in yeast and fungi. Arch Microbiol 204, 79 (2022). https://doi.org/10.1007/s00203-021-02723-7
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DOI: https://doi.org/10.1007/s00203-021-02723-7