Abstract
The diverse function of the moonlighting proteins in Acinetobacter baumannii is highly associated with its virulence that had spurred renewed attention in recent years. The existing and newly formed hypothetical moonlighting proteins, evolve without jeopardizing the structural constraints of their original roles. It is yet uncertain and undefined to lucidly describe the functions of the moonlighting proteins in A. baumannii albeit its overwhelming evidences on few proteins. This commentary thus highlights the expression and occurrence of potent moonlighting proteins in A. baumannii, rendering virulence to the strains and the reasons to target the same portraying an active arena of research.
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References
Bentancor LV, Camacho-Peiro A, Bozkurt-Guzel C, Pier GB, Maira-Litrán T (2012) Identification of Ata, a multifunctional trimeric autotransporter of Acinetobacter baumannii. J Bacteriol 194(15):3950–3960. https://doi.org/10.1128/JB.06769-11
Caldas TD, El Yaagoubi A, Richarme G (1998) Chaperone properties of bacterial elongation factor EF-Tu. J Biol Chem 273(19):11478–11482. https://doi.org/10.1074/jbc.273.19.11478
Coleman JL, Sellati TJ, Testa JE, Kew RR, Furie MB, Benach JL (1995) Borrelia burgdorferi binds plasminogen, resulting in enhanced penetration of endothelial monolayers. Infect Immun 63(7):2478–2484. https://doi.org/10.1128/iai.63.7.2478-2484.1995
Copley SD (2014) An evolutionary perspective on protein moonlighting. Biochem Soc Trans 42(6):1684–1691. https://doi.org/10.1042/BST20140245
Defeu Soufo HJ, Reimold C, Linne U, Knust T, Gescher J, Graumann PL (2010) Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein. Proc Natl Acad Sci USA 107(7):3163–3168. https://doi.org/10.1073/pnas.0911979107
Dijkshoorn L, Nemec A, Seifert H (2007) An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol 5:939–951. https://doi.org/10.1038/nrmicro1789
Girija ASS, Shoba G, Priyadharsini JV (2021) Accessing the T-cell and B-cell immuno-dominant peptides from Acinetobacter baumannii biofilm associated protein (bap) as vaccine candidates: a computational approach. Int J Pept Res Ther 27:37–45. https://doi.org/10.1007/s10989-020-10064-0
Gribun A, Nitzan Y, Pechatnikov I, Hershkovits G, Katcoff DJ (2003) Molecular and structural characterization of the HMP-AB gene encoding a pore-forming protein from a clinical isolate of Acinetobacter baumannii. Curr Microbiol 47(5):434–443. https://doi.org/10.1007/s00284-003-4050-4
Gründel A, Friedrich K, Pfeiffer M, Jacobs E, Dumke R (2015) Subunits of the pyruvate dehydrogenase cluster of Mycoplasma pneumoniae are surface-displayed proteins that bind and activate human plasminogen. PLoS ONE 10(5):e0126600. https://doi.org/10.1371/journal.pone.0126600
Harvey KL, Jarocki VM, Charles IG, Djordjevic SP (2019) The diverse functional roles of elongation factor Tu (EF-Tu) in microbial pathogenesis. Front Microbiol 10:2351. https://doi.org/10.3389/fmicb.2019.02351
Henderson B, Martin A (2011) Bacterial virulence in the moonlight: multitasking bacterial moonlighting proteins are virulence determinants in infectious disease. Infect Immun 79(9):3476–3491. https://doi.org/10.1128/IAI.00179-11
Jawad A, Seifert H, Snelling AM, Heritage J, Hawkey PM (1998) Survival of Acinetobacter baumannii on dry surfaces: comparison of outbreak and sporadic isolates. J Clin Microbiol 36(7):1938–1941. https://doi.org/10.1128/JCM.36.7.1938-1941.1998
Koenigs A, Zipfel PF, Kraiczy P (2015) Translation elongation factor Tuf of Acinetobacter baumannii is a plasminogen-binding protein. PLoS ONE 10(7):e0134418. https://doi.org/10.1371/journal.pone.0134418
Koenigs A, Stahl J, Averhoff B, Göttig S, Wichelhaus TA, Wallich R, Zipfel PF, Kraiczy P (2016) CipA of Acinetobacter baumannii Is a novel plasminogen binding and complement inhibitory protein. J Infect Dis 213(9):1388–1399. https://doi.org/10.1093/infdis/jiv601
Kunert A, Losse J, Gruszin C, Hühn M, Kaendler K, Mikkat S, Volke D, Hoffmann R, Jokiranta TS, Seeberger H, Moellmann U, Hellwage J, Zipfel PF (2007) Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein. J Immunol 179(5):2979–2988. https://doi.org/10.4049/jimmunol.179.5.2979
Smiline Girija AS (2020) Delineating the immuno-dominant antigenic vaccine peptides against gacS-sensor kinase in Acinetobacter baumannii: an in silico investigational approach. Front Microbiol 1:2078. https://doi.org/10.3389/fmicb.2020.02078
Smith DR, Chapman MR (2010) Economical evolution: microbes reduce the synthetic cost of extracellular proteins. Mbio 1(3):e00131-10 e00131-18. https://doi.org/10.1128/mBio.00131-10
Sogasu D, Girija ASS, Gunasekaran S, Priyadharsini JV (2021) Molecular characterization and epitope-based vaccine predictions for ompA gene associated with biofilm formation in multidrug-resistant strains of A. baumannii. In Silico Pharmacol 9(1):15. https://doi.org/10.1007/s40203-020-00074-7
Sugawara E, Nikaido H (2012) OmpA is the principal nonspecific slow porin of Acinetobacter baumannii. J Bacteriol 194:4089–4096. https://doi.org/10.1128/JB.00435-12
Tipton KA, Rather PN (2017) An ompR-envZ two-component system ortholog regulates phase variation osmotic tolerance, motility, and virulence in Acinetobacter baumannii strain AB5075. J Bacteriol 199(3):e00705-e716. https://doi.org/10.1128/JB.00705-16
Towner KJ (2009) Acinetobacter: an old friend, but a new enemy. J Hosp Infect 73(4):355–363. https://doi.org/10.1016/j.jhin.2009.03.032
Vanden Bergh P, Heller M, Braga-Lagache S, Frey J (2013) The Aeromonas salmonicida subsp. salmonicida exoproteome: global analysis, moonlighting proteins and putative antigens for vaccination against furunculosis. Proteome Sci 11:1–12. https://doi.org/10.1186/1477-5956-11-44
Viale AM, Evans BA (2020) Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii. Microb Genom 6(6):e000381. https://doi.org/10.1099/mgen.0.000381
Vieira ML, Alvarez-Flores MP, Kirchgatter K, Romero EL, Alves IJ, de Morais ZM, Vasconcellos SA, Chudzinski-Tavassi AM, Nascimento ALTO (2013) Interaction of Leptospira interrogans with human proteolytic systems enhances dissemination through endothelial cells and protease levels. Infect Immun 81:1764–1774. https://doi.org/10.1128/IAI.00020-13
Weidensdorfer M, Ishikawa M, Hori K, Linke D, Djahanschiri B, Iruegas R, Ebersberger I, Riedel-Christ S, Enders G, Leukert L, Kraiczy P, Rothweiler F, Cinatl J, Berger J, Hipp K, Kempf VAJ, Göttig S (2019) The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii. Virulence 10(1):68–81. https://doi.org/10.1080/21505594.2018.1558693
Xolalpa W, Vallecillo AJ, Lara M, Mendoza-Hernandez G, Comini M, Spallek R, Singh M, Espitia C (2007) Identification of novel bacterial plasminogen-binding proteins in the human pathogen Mycobacterium tuberculosis. Proteomics 7(18):3332–3341. https://doi.org/10.1002/pmic.200600876
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Dr. A.S. Smiline Girija and Dr. P. Sankar Ganesh declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Smiline Girija, A.S., Ganesh, P.S. Virulence of Acinetobacter baumannii in proteins moonlighting. Arch Microbiol 204, 96 (2022). https://doi.org/10.1007/s00203-021-02721-9
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DOI: https://doi.org/10.1007/s00203-021-02721-9