Abstract
Carbapenems, as the “last line of defense” against Gram-negative bacteria, are increasingly being challenged by drug-resistant bacteria, especially Enterobacteriaceae. In this study, a carbapenem-resistant Gram-negative bacterium, named AH001, was isolated from hospital sewage, and a modified Hodge test confirmed that this bacterium can produce carbapenemase. Further analysis revealed that this bacterium exhibits multidrug resistance against an additional seven antibiotics. Whole-genome sequencing and analysis showed that AH001 could not be classified by existing MLST, and its serotype could not be distinguished among O9, O89 or O168 according to O antigen prediction. More attention should be given to the role of environmental sources of Escherichia coli in the development and transfer of drug resistance in the hospital environment.
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Funding
This study was funded by Jiangsu Modern Agriculture (Waterfowl) Industrial Technology System Disease Prevention, Control Innovation Team JATS[2018]222, the Key research and development plan of Jiangsu province (BE2019304) and the Study on the Target Screening of Vigorous Phage and Its Prevention and Control in Colibacillosis from the National Natural Science Foundation of China, 2019.01–2021.12 U1803109.
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This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JACDTS000000000. The version described in this paper is version JACDTS000000000. The SUBID is SUB7768533.
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Communicated by Erko Stackebrandt.
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Hu, Z., Chen, W., Guo, G. et al. An Escherichia coli isolate from hospital sewage carries blaNDM-1 and blaoxa-10. Arch Microbiol 203, 4427–4432 (2021). https://doi.org/10.1007/s00203-021-02431-2
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DOI: https://doi.org/10.1007/s00203-021-02431-2