Abstract
The optimization of the bacterium Pseudomonas stutzeri SPM-1, obtained from textile wastewater dumping sites of Surat, Gujarat was studied for the degradation of the textile azo dye Procion Red—H3B. The strain showed significant activities of azoreductase (95%), laccase (76%) and NADH-DCIP reductase (88%) at 12, 10 and 8 h of growth, respectively, indicating the evidence for reductive cleavage of the dye. The optimization was carried on phenanthrene enrichment medium followed by exposing it to variable environmental factors and nutritional sources. The complete decolourization of dye (50 mg/L) happened within 20 h of incubation at pH 8 and temperature 32 ± 0.2 °C under microaerophilic condition. Decolourization was monitored with the shifting of absorbance peak in UV–Vis spectrophotometry and HPLC analysis. The changes in the functional groups were confirmed by the presence of new peaks in FT-IR data. GC–MS analysis helped in recognizing the degraded dye compounds thus elucidating the proposed pathway for Procion Red—H3B. The potential of bioremediation process was completed by phytotoxicity test using two plants Vigna radiata and Cicer arietinum. Our study concludes that the strain Pseudomonas stutzeri SPM-1, with its rapid decolourization efficiency holds noteworthy prospective in industrial application for textile wastewater treatment.
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The authors are thankful to all faculty members and non-teaching staff of the Department of Bioscience, Veer Narmad South Gujarat University, Surat, Gujarat, India for providing infrastructural facilities and active moral support towards completion of this work.
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SPB: Designing, acquisition, analysis and interpretation of data, drafting the article, reviewing it critically for significant intellectual content. SKT: Final approval of the version to be submitted for any revised version.
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Bera, S.P., Tank, S.K. Bioremedial approach of Pseudomonas stutzeri SPM-1 for textile azo dye degradation. Arch Microbiol 203, 2669–2680 (2021). https://doi.org/10.1007/s00203-021-02258-x
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DOI: https://doi.org/10.1007/s00203-021-02258-x