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Paenibacillus turpanensis sp. nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China

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Abstract

Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5–8.0. The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids. The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid. The phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unknown lipids. The DNA G + C content of the type strain was 50.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus. The nearest relatives were P. residui MC-246T and P. senegalensis JC66T, with 93.2% and 92.8% gene sequence similarities, respectively. On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp. nov. is proposed. The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).

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Abbreviations

NFA:

Nitrogen Fixing agar

TSA:

Tryptic soy agar

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PE:

Phosphatidylethanolamine

PL:

Unknown phospholipid

GL:

Unidentified glycolipids

UL:

Unknown lipids

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Acknowledgements

This study was performed with the support of the National Natural Science Foundation of China (31760003, 31500011 and 31270055), the Natural Science Foundation of Yunnan Province (2017FB039) and the South and Southeast Asia Cooperation Base on Microbiological Resource Prevention and Utilization (2018IA100)

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Author notes

  1. Li Yang and Hua-Wei Huang equally contributed to this work.

Authors and Affiliations

  1. Key Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of MicrobiologySchool of Life SciencesYunnan University, Kunming, 650091, People’s Republic of China

    Li Yang, Hua-Wei Huang, Yu Li, Xue-Qian Wang, Gui-Fang Zhao, Wen-Yong Zhu & Shu-Kun Tang

  2. Yunnan Key Laboratory of Vaccine Research and Development on Severe Infections Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, People’s Republic of China

    Wen-Yong Zhu

  3. School of Medicine, Yunnan University, Kunming, 650091, People’s Republic of China

    Min Yin

  4. Life Science and Technology School, Lingnan Normal University, Zhanjiang, 524048, People’s Republic of China

    Yun Wang

  5. Walvax Biotechnology Co., Ltd, Kunming, 650106, People’s Republic of China

    Ya-Rong Kou

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  1. Li Yang
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Contributions

LY and H-WH: carried out the data analysis, part of chemical classification and wrote the manuscript. YW, Y-RK and MY: performed the polyphasic taxonomy except chemical classification. YL, X-QW and G-FZ: prepared the experiments and isolated the novel strain. W-YZ and S-KT: designed the separation medium and directed the classification and takes full responsibility for the final submission. All the authors reviewed and approved the final version of the paper.

Corresponding authors

Correspondence to Wen-Yong Zhu or Shu-Kun Tang.

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Additional information

Communicated by Erko Stackebrandt.

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The NCBI GenBank accession number for the 16S rRNA gene sequence of strain YIM B00363T is MT032315. The draft whole-genome sequence for YIM B00363T has been deposited at DDBJ/ENA/GenBank under accession number WTLI00000000.

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Yang, L., Huang, HW., Wang, Y. et al. Paenibacillus turpanensis sp. nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China. Arch Microbiol 203, 77–83 (2021). https://doi.org/10.1007/s00203-020-02003-w

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