Abstract
A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 21826T (98.5%) and N. galegae HAMBI 540T (98.1%). Phylogenetic analysis based on 16S rRNA genes and multilocus sequence analysis (MLSA) based on the partial sequences of atpD-glnII-glnA-recA-ropD-thrC housekeeping genes both indicated that strain 24NRT is a member of the genus Neorhizobium. The average nucleotide identity between the genome sequence of strain 24NRT and that of the isolate N. alkalisoli DSM 21826T was 83.1%, and the digital DNA–DNA hybridization was 20.1%. The G+C content of strain 24NRT was 60.3 mol %. The major cellular fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c. Based on phenotypic, phylogenetic, and genotypic data, strain 24NRT is considered to represent a novel species of the genus Neorhizobium, for which the name Neorhizobium lilium sp. nov. is proposed. The type strain is 24NRT (= ACCC 61588T = JCM 33731T).
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Notes
The GenBank accession number for the 16S rRNA gene sequence of strain 24NRT is MK386721. The accession number for the draft genome sequence of strain 24NRT is GCA_004053875.1. The Digital Protologue database TaxoNumber for strain 24NRT isTA00988.
Abbreviations
- ANI:
-
Average nucleotide identity
- GGDC:
-
Genome-to-genome distance calculator
- dDDH:
-
Digital DNA–DNA hybridization
- MEGA:
-
Molecular evolutionary genetics analysis
- MLSA:
-
Multilocus sequence analysis
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Acknowledgements
The authors are grateful to Prof. Jianguan Sun (Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences) and Prof. Junlian Gao (Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology) for their help in supporting the strain Rhizobium wenxiniae CGMCC 1.15279T.
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The Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018SY004).
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Lei Liu conceived the study, participated in its design, and coordinated and drafted the manuscript; Shaowen Shi, Lijun Xu, and Miao Chi participated in the design and coordination of the study and performed the measurement; Xiaoxiao Zhang and Lubin Li conceived the study, participated in its design, and coordinated and drafted the manuscript. All authors read and approved the final manuscript.
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Communicated by Erko Stackebrandt.
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Liu, L., Shi, S., Liang, L. et al. Neorhizobium lilium sp. nov., an endophytic bacterium isolated from Lilium pumilum bulbs in Hebei province. Arch Microbiol 202, 609–616 (2020). https://doi.org/10.1007/s00203-019-01774-1
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DOI: https://doi.org/10.1007/s00203-019-01774-1