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Cystobasidium keelungensis sp. nov., a novel mycosporine producing carotenogenic yeast isolated from the sea surface microlayer in Taiwan

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Abstract

Cystobasidium keelungensis SN2T (CBS 6949 = BCRC 920080), a new anamorphic basidiomycetous yeast species, is described in this paper. The strains belonging to this species were isolated from the sea surface microlayer and underlying water in Taiwan. These strains were identified by examining nucleotide sequences in the species-specific D1/D2 domains of the large subunit (LSU) ribosomal RNA (rRNA) and by evaluating their physiological characteristics. Phylogenetic analyses of D1/D2 sequences revealed that C. keelungensis was most closely related to the species C. slooffiae (LSU rRNA gene sequence divergence of 1.5%), and it belonged to the Erythrobasidium clade. No sexual reproduction was observed. This species differed from related species in carbon and nitrogen assimilation patterns and growth at 35 °C. Screening C. keelungensis for the presence of UV-absorbing compounds showed that mycosporine–glutaminol–glucoside and mycosporine–glutamicol–glucoside (maximum absorption: 310 nm) were the major UV-absorbing compounds, which differ from the compounds present in some freshwater yeast strains reported in previous studies. After UV induction, SN2 had a higher level of mycosporine production than other carotenogenic yeasts in this study.

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Acknowledgements

This work was supported by Grants-in-Aid from the Ministry of Science and Technology, Executive Yuan, Taiwan (MOST 105-2311-B-157-001).

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Authors and Affiliations

  1. Department of Biological Science and Technology, China University of Science and Technology, Taipei, 11581, Taiwan

    Chin-Feng Chang

  2. Department of Applied Science, National Tsing Hua University, Nanda Campus, 521 Nanda Road, Hsinchu, 30014, Taiwan

    Ching-Fu Lee

  3. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan

    Ching-Fu Lee

  4. Institute of Marine Biology, National Taiwan Ocean University, keelung, 20224, Taiwan

    Shiu-Mei Liu

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  1. Chin-Feng Chang
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  2. Ching-Fu Lee
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Correspondence to Shiu-Mei Liu.

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Communicated by Olaf Kniemeyer.

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Chang, CF., Lee, CF. & Liu, SM. Cystobasidium keelungensis sp. nov., a novel mycosporine producing carotenogenic yeast isolated from the sea surface microlayer in Taiwan. Arch Microbiol 201, 27–33 (2019). https://doi.org/10.1007/s00203-018-1570-7

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  • DOI: https://doi.org/10.1007/s00203-018-1570-7

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