Abstract
Colletotrichum gloeosporioides is the causal agent of rubber anthracnose, which is also one of the important biological factors threatening the development of natural rubber industry in the world. Regulators of G-protein signaling (RGS) are key negative regulators of G-proteins, which play important roles in growth, development and pathogenic processes of plant pathogens. In this study, a RGS gene CgRGS1 was functionally characterized in C. gloeosporioides. Compared to the wild type, the CgRGS1 deletion mutant had slow vegetative growth, reduced conidia with multi-end germination, low appressorium formation rate, high resistance to oxidative stress and SDS. Moreover, the mutant was sensitive to osmotic pressure and showed decreased virulence. In conclusion, CgRGS1 is involved in regulation of vegetative growth, conidiation, germination, appressorium formation, oxidative stress, osmotic pressure response and pathogenicity in C. gloeosporioides.
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References
Burchett SA (2000) Regulators of G protein signaling: a bestiary of modular protein binding domains. J Neurochem 75:1335–1351
Cai Z, Li G, Lin C, Shi T, Zhai L, Chen Y, Huang G (2013) Identifying pathogenicity genes in the rubber tree anthracnose fungus Colletotrichum gloeosporioides through random insertional mutagenesis. Microbiol Res 168:340–350
De Vries L, Zheng B, Fischer T, Elenko E, Farquhar MG (2000) The regulator of G protein signaling family. Annu Rev Pharmacol Toxicol 40:235–271
Dignard D, Whiteway M (2006) SST2, a regulator of G-protein signaling for the Candida albicans mating response pathway. Eukaryot Cell 5:192–202
Dohlman HG, Song JP, Ma DR, Courchesne WE, Thorner J (1996) Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein α subunit). Mol Cell Biol 16:5194–5209
Fang WG, Pei Y, Bidochka MJ (2007) A regulator of a G protein signalling (RGS) gene, cag8, from the insect-pathogenic fungus Metarhizium anisopliae is involved in conidiation, virulence and hydrophobin synthesis. Microbiology 153:1017–1025
Fang WG, Scully LR, Zhang L, Pei Y, Bidochka MJ (2008) Implication of a regulator of G protein signalling (BbRGS1) in conidiation and conidial thermotolerance of the insect pathogenic fungus Beauveria bassiana. FEMS Microbiol Lett 279:146–156
Guan KL, Han M (1999) A G-protein signaling network mediated by an RGS protein. Gene Dev 13:1763–1767
Jean G, Edith NO, Fabrice P (2005) Some epidemiological investigations on Colletotrichum leaf disease on rubber tree. Crop Prot 24:65–77
Kim YK, Kawano T, Li D, Kolattukudy PE (2000) A mitogen-activated protein kinase kinase required for Induction of cytokinesis and appressorium formation by host signals in the conidia of Colletotrichum gloeosporioides. Plant Cell 12:1331–1343
Li L, Wright SJ, Krystofova S, Park G, Borkovich KA (2007) Heterotrimeric G protein signaling in filamentous fungi. Annu Rev Microbiol 61:423–452
Lieberei R (2007) South American leaf blight of the rubber tree (Hevea spp.): new steps in plant domestication using physiological features and molecular markers. Ann Bot–London 100:125–142
Liu H, Suresh A, Willard FS, Siderovski DP, Lu S, Naqvi NI (2007) Rgs1 regulates multiple Gα subunits in Magnaporthe pathogenesis, asexual growth and thigmotropism. EMBO J 26:690–700
McCabe MS, Power JB, de Laat AM, Davey MR (1997) Detection of single-copy genes in DNA from transgenic plants by nonradioactive Southern blot analysis. Mol Biotechnol 7:79–84
Mukherjee M, Kim JE, Park YS, Kolomiets MV, Shim WB (2011) Regulators of G-protein signalling in Fusarium verticillioides mediate differential host–pathogen responses on nonviable versus viable maize kernels. Mol Plant Pathol 12:479–491
Park AR, Cho AR, Seo JA, Min K, Son H, Lee J, Choi GJ, Kim JC, Lee YW (2012) Functional analyses of regulators of G protein signaling in Gibberella zeae. Fungal Genet Biol 49:511–520
Priyatno TP, Bakar FDA, Kamaruddin N, Mahadi NM, Abdul Murad AM (2012) Inactivation of the catalytic subunit of cAMP-dependent protein kinase A causes delayed appressorium formation and reduced pathogenicity of Colletotrichum gloeosporioides. Sci World J (ID 545784)
Shelp BJ, Bown AW, McLean MD (1999) Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci 4:446–452
Siderovski DP, Willard FS (2005) The GAPs, GEFs, and GDIs of heterotrimeric G-Protein alpha subunits. Int J Bio Sci 1:51–66
Talbot NJ, Ebbole DJ, Hamer JE (1993) Identification and characterization of MPGI, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5:1575–1590
Wang Y, Geng Z, Jiang D, Long F, Zhao Y, Su H, Zhang KQ, Yang J (2013) Characterizations and functions of regulator of G protein signaling (RGS) in fungi. Appl Microbiol Biotechnol 97:7977–7987
Xu X, Wang Y, Tian C, Liang Y (2016) The Colletotrichum gloeosporioides RhoB regulates cAMP and stress response pathways and is required for pathogenesis. Fungal Genet Biol 96:12–24
Xue C, Hsueh YP, Chen L, Heitman J (2008) The RGS protein Crg2 regulates both pheromone and cAMP signalling in Cryptococcus neoformans. Mol Microbiol 70:379–395
Yong HY, Bakar FD, Illias RM, Mahadi NM, Murad AM (2013) Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioides. Braz J Microbiol 44:1241–1250
Yu JH (2006) Heterotrimeric G Protein signaling and RGSs in Aspergillus nidulans. J Microbiol 44:145–154
Yu JH, Keller N (2005) Regulation of secondary metabolism in filamentous fungi. Annu Rev Phytopathol 43:437–458
Zhang H, Tang W, Liu K, Huang Q, Zhang X, Yan X, Chen Y, Wang J, Qi Z, Wang Z, Zheng X, Wang P, Zhang Z (2011) Eight RGS and RGS like proteins orchestrate growth, differentiation, and pathogenicity of Magnaporthe oryzae. PLOS Pathog 7:e1002450
Zhang N, Liu Z, Wu M, Li X (2017) Gene cloning and functional analysis of CgSho1 in Colletotrichum gloeosporioides. Acta Phytopathol Sin 47:40–49
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 31560045) and the Key Research and Development Project of Hainan (Grant No. ZDYF2016155).
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Liu, ZQ., Wu, ML., Ke, ZJ. et al. Functional analysis of a regulator of G-protein signaling CgRGS1 in the rubber tree anthracnose fungus Colletotrichum gloeosporioides . Arch Microbiol 200, 391–400 (2018). https://doi.org/10.1007/s00203-017-1455-1
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DOI: https://doi.org/10.1007/s00203-017-1455-1