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Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil

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Abstract

Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20–36 °C (optimal 28 °C), pH ranges 5–11 (optimal 6.0–7.0), and 0.1–0.5%NaCl (optimal 0.1–0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity. Multi-locus sequence analysis (MLSA) with dnaK, glnII, recA, and rpoB confirmed B. neotropicale BR 10247T as the closest type strain for the group I and B. elkanii USDA 76T and B. pachyrhizi PAC 48T for group II. Average Nucleotide Identity (ANI) differentiated group I from the B. neotropicale BR 10247T (79.6%) and group II from B. elkanii USDA 76T and B. pachyrhizi PAC 48T (88.1% and 87.9%, respectively). Fatty acid profiles [majority C16:0 and Summed feature 8 (18:1ω6c/18:1ω7c) for both groups], DNA G + C content, and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae, and Phaseoleae. Based on the presented data, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed, with BR 10245T (=HAMBI 3597T) and BR 10303T (=HAMBI 3602T) as the respective-type strains.

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Acknowledgements

The authors would like to thank Rosa Pitard, Fernanda Dourado, Natalia Camacho, and Karine Freitas (Embrapa Agrobiologia) for technical assistance. Embrapa, CNPq, and FAPERJ financially supported this study.

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Authors and Affiliations

  1. Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23890-000, Brazil

    Daniele C. Michel, Samuel R. Passos & Alexandre C. Baraúna

  2. Embrapa Agrobiologia, Rodovia BR 465 km 07, Seropédica, Rio De Janeiro, 23891-000, Brazil

    Jean L. Simões-Araujo & Jerri E. Zilli

  3. Embrapa Roraima, Rodovia BR 174 km 08, Boa Vista, Roraima, 69301-970, Brazil

    Krisle da Silva

  4. Embrapa Meio Ambiente, Rodovia SP 340 km 127, 5, Jaguariúna, São Paulo, 13820-000, Brazil

    Marcia M. Parma & Itamar S. Melo

  5. Centre for Rhizobium Studies, Murdoch University, 90 South Street, Murdoch, WA, 6150, Australia

    Sofie E. De Meyer & Graham O’Hara

Authors
  1. Daniele C. Michel
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  2. Samuel R. Passos
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  3. Jean L. Simões-Araujo
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  4. Alexandre C. Baraúna
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  5. Krisle da Silva
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  6. Marcia M. Parma
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  7. Itamar S. Melo
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  8. Sofie E. De Meyer
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  9. Graham O’Hara
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  10. Jerri E. Zilli
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Correspondence to Jerri E. Zilli.

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Communicated by Erko Stackebrandt.

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Michel, D.C., Passos, S.R., Simões-Araujo, J.L. et al. Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil. Arch Microbiol 199, 657–664 (2017). https://doi.org/10.1007/s00203-017-1340-y

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  • DOI: https://doi.org/10.1007/s00203-017-1340-y

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