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Hopanoid inventory of Rhodoplanes spp.

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Abstract

Hopanoids are pentacyclic triterpenoid lipids and are important for bacterial membrane stability and functioning. These pentacyclic triterpenoids of hopane series are biomarkers for eubacteria and can be used as chemotaxonomic markers. Anoxygenic phototrophic bacteria are good producers of hopanoids, and their inventory to date is restricted to a few members. Rhodoplanes spp. are phototrophic prokaryotes which grow and thrive in subsurface and sediment environments. A study on the diversity of hopanoids of several species of Rhodoplanes revealed a rich diversity of hopanoids with carbon length of C30/C31 and C35. Hop-22(29)-ene (II), diplopterol (V), tetrahymanol (VII), 2-methyldiplopterol (VI), 2-methyltetrahymanol (VIII), bacteriohopanetetrol (IX), bacteriohopaneaminotriol (X) and bacteriohopanepolyols [BHP-492 (XIII), BHP-550 (XIV), BHP-508 (XII)] are the major hopanoids of the genus Rhodoplanes. Tetrahymanol (VII) content is high (38–60 %) among all the members, except for Rhodoplanes elegans. Hopanoid fingerprints allowed differentiation of species of the genus Rhodoplanes. Statistical analyses also indicate hopanoids as good chemotaxonomic markers to distinguish species of the genus Rhodoplanes.

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Acknowledgments

This work was funded by Council of Scientific and Industrial Research (CSIR). TL is supported by research fellowship from the University Grants Commission (UGC). SA is supported by research fellowship from the CSIR-SRF. Infrastructural facility support provided by DST as FIST is greatly acknowledged.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India

    Tushar Dilipchand Lodha & Chintalapati Venkata Ramana

  2. Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad, 500 085, India

    Are Srinivas & Chintalapati Sasikala

Authors
  1. Tushar Dilipchand Lodha
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  2. Are Srinivas
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  3. Chintalapati Sasikala
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  4. Chintalapati Venkata Ramana
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Correspondence to Chintalapati Venkata Ramana.

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Communicated by Erko Stackebrandt.

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Lodha, T.D., Srinivas, A., Sasikala, C. et al. Hopanoid inventory of Rhodoplanes spp.. Arch Microbiol 197, 861–867 (2015). https://doi.org/10.1007/s00203-015-1112-5

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  • DOI: https://doi.org/10.1007/s00203-015-1112-5

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