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Strain-dependent response to Cu2+ in the expression of laccase in Pycnoporus coccineus

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Abstract

The effects of Cu2+ on the activity and expression of laccase were investigated in seven different strains of Pycnoporus coccineus collected from different regions in Korea. Cu2+ was toxic to mycelial growth at concentrations greater than 0.5 mM CuSO4 and showed complete growth inhibition at 1 mM in the liquid culture. However, Cu2+ significantly upregulated the extracellular laccase activity at 0.2 mM in five strains of P. coccineus, IUM4209, IUM0032, IUM0450, IUM0470, and IUM4093, whereas two strains, IUM0253 and IUM0049, did not respond to Cu2+, despite being closely related to the other five strains. Subsequent RT-PCR analysis also showed that the laccase mRNA was highly expressed only in the former five strains in the presence of Cu2+. Taken together, these results indicate that Cu2+ regulates expression of the laccase gene in a strain-dependent manner. The five strains commonly produced a single predominant laccase protein with a molecular weight of 68 kDa. Peptide sequencing revealed that the laccase was a homolog of Lcc1 of P. coccineus, which was isolated in China. The Cu2+-induced culture supernatants exhibited high degradation of polycyclic aromatic hydrocarbons, indicating that the 68-kDa laccase is the primary extracellular degradative enzyme in P. coccineus.

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Acknowledgments

This study was supported by “The Genetic Evaluation of Important Biological Resources” grant funded by the National Institute of Biological Resources, Korea. JWP, BSH, and SK were supported by a scholarship from the BK 21 Plus Program, maintained by the Ministry of Education, Korea.

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Authors and Affiliations

  1. Division of Applied Life Science and Research Institute for Life Science, Gyeongsang National University, Jinju, 660-701, Korea

    Ju-Wan Park, Hyeon-Woo Kang, Byung-Suk Ha, Sin-Il Kim & Hyeon-Su Ro

  2. National Institute of Biological Resources, Incheon, 404-708, Korea

    Soonok Kim

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  1. Ju-Wan Park
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  2. Hyeon-Woo Kang
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Correspondence to Hyeon-Su Ro.

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Communicated by Olaf Kniemeyer.

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Park, JW., Kang, HW., Ha, BS. et al. Strain-dependent response to Cu2+ in the expression of laccase in Pycnoporus coccineus . Arch Microbiol 197, 589–596 (2015). https://doi.org/10.1007/s00203-015-1090-7

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