Abstract
Newborns acquire their first microbiota at birth. Maternal vaginal or skin bacteria colonize newborns delivered vaginally or by C-section, respectively (Dominguez-Bello et al. 2010 #884). We aimed to determine differences in the presence of four tetracycline (tet) resistance genes, in the microbes of ten newborns and in the mouth and vagina of their mothers, at the time of birth. DNA was amplified by PCR with primers specific for [tet(M), tet(O), tet(Q), and tet(W)]. Maternal vaginas harbored all four tet resistance genes, but most commonly tet(M) and tet(O) (63 and 38 %, respectively). Genes coding for tet resistance differed by birth mode, with 50 % of vaginally delivered babies had tet(M) and tet(O) and 16 and 13 % of infants born by C-section had tet(O) and tet(W), respectively. Newborns acquire antibiotic resistance genes at birth, and the resistance gene profile varies by mode of delivery.
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Acknowledgments
We acknowledge the support of the programs for undergraduate minorities at University of Puerto Rico, The Puerto Rico Louis Strokes Alliance for Minority Participation and BioMinds to AMAS, and the UPR NIH SCoRE PES program.
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Communicated by Erko Stackebrandt.
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Alicea-Serrano, A.M., Contreras, M., Magris, M. et al. Tetracycline resistance genes acquired at birth. Arch Microbiol 195, 447–451 (2013). https://doi.org/10.1007/s00203-012-0864-4
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DOI: https://doi.org/10.1007/s00203-012-0864-4