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Production and function of jasmonates in nodulated roots of soybean plants inoculated with Bradyrhizobium japonicum

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Abstract

Little is known regarding production and function of endogenous jasmonates (JAs) in root nodules of soybean plants inoculated with Bradyrhizobium japonicum. We investigated (1) production of jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) in roots of control and inoculated plants and in isolated nodules; (2) correlations between JAs levels, nodule number, and plant growth during the symbiotic process; and (3) effects of exogenous JA and OPDA on nodule cell number and size. In roots of control plants, JA and OPDA levels reached a maximum at day 18 after inoculation; OPDA level was 1.24 times that of JA. In roots of inoculated plants, OPDA peaked at day 15, whereas JA level did not change appreciably. Shoot dry matter of inoculated plants was higher than that of control at day 21. Chlorophyll a decreased more abruptly in control plants than in inoculated plants, whereas b decreased gradually in both cases. Exogenous JA or OPDA changed number and size of nodule central cells and peripheral cells. Findings from this and previous studies suggest that increased levels of JA and OPDA in control plants are related to senescence induced by nutritional stress. OPDA accumulation in nodulated roots suggests its involvement in “autoregulation of nodulation.”

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Abbreviations

AOC:

Allene oxide cyclase

AON:

Autoregulation of nodulation

AOS:

Allene oxide synthase

DM:

Dry matter

JA:

Jasmonic acid

JAs:

Jasmonates

LOX:

Lipoxygenase

MeJA:

Methyl-jasmonate

OPDA:

12-Oxophytodienoic acid

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Acknowledgments

This work was supported by grants from SECYT-UNRC and ANPCYT to G.A. The authors thank Dr. S. Anderson for English editing.

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Authors and Affiliations

  1. Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800, Río Cuarto, Córdoba, Argentina

    María Emilia Costanzo, Andrea Andrade, María del Carmen Tordable, Fabricio Cassán & Guillermina Abdala

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  1. María Emilia Costanzo
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  2. Andrea Andrade
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  3. María del Carmen Tordable
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  4. Fabricio Cassán
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  5. Guillermina Abdala
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Correspondence to Fabricio Cassán.

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Communicated by Ursula Priefer.

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Costanzo, M.E., Andrade, A., del Carmen Tordable, M. et al. Production and function of jasmonates in nodulated roots of soybean plants inoculated with Bradyrhizobium japonicum . Arch Microbiol 194, 837–845 (2012). https://doi.org/10.1007/s00203-012-0817-y

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  • DOI: https://doi.org/10.1007/s00203-012-0817-y

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