Abstract
Rhodobacter sphaeroides ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-deletion strain 16 was capable of photoheterotrophic growth with acetate, while Rhodopseudomonas palustris RubisCO-deletion strain 2040 could not grow under these conditions. The reason for this difference lies in the fact that Rba. sphaeroides and Rps. palustris use different pathways for acetate assimilation, the ethylmalonyl-CoA pathway, and glyoxylate-bypass cycle, respectively. The ethylmalonyl-CoA pathway is distinct from the glyoxylate cycle as one molecule of CO2 and one molecule of HCO3− per three molecules of acetyl-CoA are co-assimilated to form two malate molecules. The glyoxylate cycle directly converts two acetyl-CoA molecules to malate. Each pathway, therefore, also dictates at what point, CO2 and reductant are consumed, thereby determining the requirement for the Calvin–Benson–Bassham reductive pentose phosphate cycle.
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Acknowledgments
We thank Trung Ho for assistance with the growth experiments. This work was supported by grants of the National Science Foundation MCB0842892, by a start-up fund from the Ohio State University (B. E. A.), and by grants DE-FG02-07ER64489 and DE-FG02-DE-FG02-08ER15976 from the Office of Biological and Environmental Research (Genomics: GTL Program) and the Chemical Sciences, Geosciences, and Biosciences Division of the Office of Basic Energy Sciences, respectively, of the U.S. Department of Energy (F. R. T.).
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Communicated by Theo Hansen.
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Laguna, R., Tabita, F.R. & Alber, B.E. Acetate-dependent photoheterotrophic growth and the differential requirement for the Calvin–Benson–Bassham reductive pentose phosphate cycle in Rhodobacter sphaeroides and Rhodopseudomonas palustris . Arch Microbiol 193, 151–154 (2011). https://doi.org/10.1007/s00203-010-0652-y
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DOI: https://doi.org/10.1007/s00203-010-0652-y