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Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid β-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria

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An Erratum to this article was published on 06 May 2011

Abstract

We screened for microorganisms able to use flavonoids as a carbon source; and one isolate, nominated Stilbella fimetaria SES201, was found to possess a disaccharide-specific hydrolase. It was a cell-bound ectoenzyme that was released to the medium during conidiogenesis. The enzyme was shown to cleave the flavonoid hesperidin (hesperetin 7-O-α-rhamnopyranosyl-β-glucopyranoside) into rutinose (α-rhamnopyranosyl-β-glucopyranose) and hesperetin. Since only intracellular traces of monoglycosidase activities (β-glucosidase, α-rhamnosidase) were produced, the disaccharidase α-rhamnosyl-β-glucosidase was the main system utilized by the microorganism for hesperidin hydrolysis. The enzyme was a glycoprotein with a molecular weight of 42224 Da and isoelectric point of 5.7. Even when maximum activity was found at 70°C, it was active at temperatures as low as 5°C, consistent with the psychrotolerant character of S. fimetaria. Substrate preference studies indicated that the enzyme exhibits high specificity toward 7-O-linked flavonoid β-rutinosides. It did not act on flavonoid 3-O-β-rutinoside and 7-O-β-neohesperidosides, neither monoglycosylated substrates. In an aqueous medium, the α-rhamnosyl-β-glucosidase was also able to transfer rutinose to other acceptors besides water, indicating its potential as biocatalyst for organic synthesis. The monoenzyme strategy of S. fimetaria SES201, as well as the enzyme substrate preference for 7-O-β-flavonoid rutinosides, is unique characteristics among the microbial flavonoid deglycosylation systems reported.

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Acknowledgments

This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Pampa (UNLPam) and Agencia Nacional de Promoción Científica y Técnica (ANPCyT) of Argentina. The authors gratefully thank the contributions of Eduardo Piontelli, Jorge Oyhenart and Alejandra Martínez in strain identification, Martin Hedström for mass spectrometry analysis and María Rita Martearena, Elsa Scaroni and Mirta Daz for the generous gift of flavonoids. Finally, we are indebted to Maria Andersson for helpful suggestions and critical reading of the manuscript.

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  1. CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Departamento de Química, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa (UNLPam), Av. Uruguay 151, 6300, Santa Rosa, La Pampa, Argentina

    Laura Mazzaferro, Lucrecia Piñuel, Marisol Minig & Javier D. Breccia

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  1. Laura Mazzaferro
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  2. Lucrecia Piñuel
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Correspondence to Javier D. Breccia.

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Communicated by Erko Stackebrandt.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00203-011-0709-6

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Mazzaferro, L., Piñuel, L., Minig, M. et al. Extracellular monoenzyme deglycosylation system of 7-O-linked flavonoid β-rutinosides and its disaccharide transglycosylation activity from Stilbella fimetaria . Arch Microbiol 192, 383–393 (2010). https://doi.org/10.1007/s00203-010-0567-7

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