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Mixotrophic metabolism in Burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of Burkholderia kururiensis as Burkholderia kururiensis subsp. kururiensis subsp. nov.

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Abstract

A thiosulfate-oxidizing facultative chemolithoautotrophic Burkholderia sp. strain ATSB13T was previously isolated from rhizosphere soil of tobacco plant. Strain ATSB13T was aerobic, Gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. Strain ATSB13T exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. A phylogenetic study based on 16S rRNA gene sequence analysis indicated that strain ATSB13T was most closely related to Burkholderia kururiensis KP23T (98.7%), Burkholderia tuberum STM678T (96.5%) and Burkholderia phymatum STM815T (96.4%). Chemotaxonomic data [G+C 64.0 mol%, major fatty acids, C18:1 ω7c (28.22%), C16:1 ω7c/15 iso 2OH (15.15%), and C16:0 (14.91%) and Q-8 as predominant respiratory ubiquinone] supported the affiliation of the strain ATSB13T within the genus Burkholderia. Though the strain ATSB13T shared high 16S rRNA gene sequence similarity with the type strain of B. kururiensis but considerably distant from the latter in terms of several phenotypic and chemotaxonomic characteristics. DNA–DNA hybridization between strain ATSB13T and B. kururiensis KP23T was 100%, and hence, it is inferred that strain ATSB13T is a member of B. kururiensis. On the basis of data obtained from this study, we propose that B. kururiensis be subdivided into B. kururiensis subsp. kururiensis subsp. nov. (type strain KP23T = JCM 10599T = DSM 13646T) and B. kururiensis subsp. thiooxydans subsp. nov. (type strain ATSB13T = KACC 12758T).

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Acknowledgments

RA thanks Rural Development Administration (RDA), Republic of Korea for awarding post doctoral fellowship. This study was supported by National Academy of Agricultural Science, RDA, Republic of Korea.

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Authors and Affiliations

  1. Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea

    Rangasamy Anandham, Yong Ki Kim & Hyeong Jin Jee

  2. Department of Agricultural Chemistry, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Pandiyan Indira Gandhi & Tong Min Sa

  3. Korean Agricultural Culture Collection (KACC) , Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea

    Soon Wo Kwon

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  1. Rangasamy Anandham
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  4. Tong Min Sa
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Correspondence to Rangasamy Anandham or Tong Min Sa.

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Communicated by Erko Stackebrandt.

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Anandham, R., Indira Gandhi, P., Kwon, S.W. et al. Mixotrophic metabolism in Burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of Burkholderia kururiensis as Burkholderia kururiensis subsp. kururiensis subsp. nov.. Arch Microbiol 191, 885–894 (2009). https://doi.org/10.1007/s00203-009-0517-4

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