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Cyclic AMP regulates the biosynthesis of cellobiohydrolase in Cellulomonas flavigena growing in sugar cane bagasse

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Abstract

Cellulomonas flavigena produces a battery of cellulase components that act concertedly to degrade cellulose. The addition of cAMP to repressed C. flavigena cultures released catabolic repression, while addition of cAMP to induced C. flavigena cultures led to a cellobiohydrolase hyperproduction. Exogenous cAMP showed positive regulation on cellobiohydrolase production in C. flavigena grown on sugar cane bagasse. A C. flavigena cellobiohydrolase gene was cloned (named celA), which coded for a 71- kDa enzyme. Upstream, a repressor celR1, identified as a 38 kDa protein, was monitored by use of polyclonal antibodies.

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Acknowledgments

This work was supported by grant 45678-Z from Consejo Nacional de Ciencia y Tecnología (Conacyt), Mexico. JAHH was supported by PhD fellowship from Conacyt. The authors thank M. Mercado-Morales for technical assistance.

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Authors and Affiliations

  1. Department of Biotechnology and Bioengineering CINVESTAV-IPN, Av. IPN 2508, 07300, Sn. Pedro Zacatenco, México, DF, Mexico

    Jesús Antonio Herrera-Herrera, Odilia Pérez-Avalos & Teresa Ponce-Noyola

  2. Department of Biochemistry CICATA-IPN, Unidad Querétaro, Cerro Blanco 141, 076090, Colinas del Cimatario, Querétaro, Mexico

    Luis M. Salgado

Authors
  1. Jesús Antonio Herrera-Herrera
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  2. Odilia Pérez-Avalos
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  3. Luis M. Salgado
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  4. Teresa Ponce-Noyola
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Corresponding author

Correspondence to Teresa Ponce-Noyola.

Additional information

Communicated by Jorge Membrillo-Hernández.

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Herrera-Herrera, J.A., Pérez-Avalos, O., Salgado, L.M. et al. Cyclic AMP regulates the biosynthesis of cellobiohydrolase in Cellulomonas flavigena growing in sugar cane bagasse. Arch Microbiol 191, 745–750 (2009). https://doi.org/10.1007/s00203-009-0502-y

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  • DOI: https://doi.org/10.1007/s00203-009-0502-y

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