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Comparative study of Bifidobacterium animalis, Escherichia coli, Lactobacillus casei and Saccharomyces boulardii probiotic properties

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Abstract

The present work investigates some probiotic properties of four different microorganisms (Bifidobacterium animalis var. lactis BB-12, Escherichia coli EMO, Lactobacillus casei and Saccharomyces boulardii). In vitro and in vivo tests were carried out to compare cell wall hydrophobicity, production of antagonistic substances, survival capacity in the gastrointestinal tract of germ-free mice without pathological consequence, and immune modulation by stimulation of Küpffer cells, intestinal sIgA and IL-10 levels. In vitro antagonism against pathogenic bacteria and yeast was only observed for the probiotic bacteria B. animalis and L. casei. The hydrophobic property of the cell wall was higher for B. animalis and E. coli EMO, and this property could be responsible for a better ability to colonize the gastrointestinal tract of germ-free mice. Higher levels of sIgA were observed mainly for S. boulardii, followed by E. coli EMO and B. animalis, and only S. boulardii induced a significant higher level of IL-10. In conclusion, for a probiotic use, S. boulardii presented better characteristics in terms of immunomodulation, and B. animalis and L. casei for antagonistic substance production. The knowledge of the different probiotic properties could be used to choice the better microorganism depending on the therapeutic or prophylactic application.

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Acknowledgments

This study was supported by grants and fellowships from the Conselho Nacional do Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG). The authors are grateful to Bernardo B. de Paula for valuable technical help and Antônio M. Vaz for animal care.

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Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, Belo Horizonte, MG, 30161-970, Brazil

    Flaviano S. Martins, Flávio H. F. Barbosa & Jacques Robert Nicoli

  2. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Flaviano S. Martins, Angélica T. Vieira & Mauro M. Teixeira

  3. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Rosa M. E. Arantes

  4. Pontifícia Universidade Católica de Minas Gerais (PUC-Minas), Belo Horizonte, MG, Brazil

    Aparecida A. Silva

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  1. Flaviano S. Martins
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  2. Aparecida A. Silva
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  3. Angélica T. Vieira
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  4. Flávio H. F. Barbosa
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  5. Rosa M. E. Arantes
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  6. Mauro M. Teixeira
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  7. Jacques Robert Nicoli
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Corresponding author

Correspondence to Jacques Robert Nicoli.

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Communicated by Erko Stackebrandt.

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Martins, F.S., Silva, A.A., Vieira, A.T. et al. Comparative study of Bifidobacterium animalis, Escherichia coli, Lactobacillus casei and Saccharomyces boulardii probiotic properties. Arch Microbiol 191, 623–630 (2009). https://doi.org/10.1007/s00203-009-0491-x

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  • DOI: https://doi.org/10.1007/s00203-009-0491-x

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