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Occurrence and antagonistic potential of Stenotrophomonas strains isolated from deep-sea invertebrates

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Abstract

Stenotrophomonas maltophilia is known to be of significance as opportunistic pathogen as well as a source of biocontrol and bioremediation activities. S. maltophilia strains have been isolated from rhizospheres, soil, clinical material, aquatic habitats, but little is known about Stenotrophomonas strains recovered from marine environments. During a survey of the biodiversity of Pseudomonas-like bacteria associated with deep-sea invertebrates six Stenotrophomonas strains were isolated from sponge, sea urchin, and ophiura specimens collected from differing Pacific areas, including the Philippine Sea, the Fiji Sea and the Bering Sea. 16S rRNA gene sequence analysis confirmed an assignment of marine isolates to the genus Stenotrophomonas as it placed four strains into the S. maltophilia CIP 60.77T cluster and two related to the S. rhizophila DSM 14405T. Together with a number of common characteristics typical of S. maltophilia and S. rhizophila marine isolates exhibited differences in pigmentation, a NaCl tolerance, a range of temperatures, which supported their growth, substrate utilization pattern, and antibiotics resistance. Strains displayed hemolytic and remarkable inhibitory activity against a number of fungal cultures and Gram-positive microorganisms, but very weak or none against Candida albicans. This is the first report on isolation, taxonomic characterization and antimicrobial activity of Stenotrophomonas strains isolated from deep-sea invertebrates.

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Acknowledgments

We would like to thank Dr. Chantal Bizet, the Collection de l’Institut Pasteur, Institut Pasteur, CIP, Paris, France, for providing the type strain of Stenotrophomonas maltophilia CIP 60.77T and indicator strains. This study was supported by a grant from the Russian Foundation for Basic Research (RFBR) and the Far-Eastern Branch of Russian Academy of Sciences (RAS) 06-04-96007, grant from the Far-Eastern Branch of RAS 06-III-A-06183, and by grant from Presidium of RAS “Molecular and Cell Biology” and grant from President of the Russian Federation “Leading Scientific School of RF (6477.2006.4)”.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Prospect 100 Let Vladivostoku, 159, Russia

    Lyudmila A. Romanenko, Galina M. Frolova, Natalia N. Slinkina & Valery V. Mikhailov

  2. Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Laboratory of Food Science and Technology, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan

    Masataka Uchino

  3. NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan

    Naoto Tanaka

Authors
  1. Lyudmila A. Romanenko
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  2. Masataka Uchino
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  3. Naoto Tanaka
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  4. Galina M. Frolova
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  5. Natalia N. Slinkina
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  6. Valery V. Mikhailov
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Correspondence to Lyudmila A. Romanenko.

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Communicated by Erko Stackebrandt.

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Romanenko, L.A., Uchino, M., Tanaka, N. et al. Occurrence and antagonistic potential of Stenotrophomonas strains isolated from deep-sea invertebrates. Arch Microbiol 189, 337–344 (2008). https://doi.org/10.1007/s00203-007-0324-8

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  • DOI: https://doi.org/10.1007/s00203-007-0324-8

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