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Regulation of expression of Na+-translocating NADH:quinone oxidoreductase genes in Vibrio harveyi and Klebsiella pneumoniae

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Abstract

The expression of genes encoding sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) was studied in the marine bacterium Vibrio harveyi and in the enterobacterium Klebsiella pneumoniae. It has been shown that such parameters as NaCl concentration, pH value, and presence of an uncoupler in the growth media do not influence significantly the level of nqr expression. However, nqr expression depends on the growth substrates used by these bacteria. Na+-NQR is highly repressed in V. harveyi during anaerobic growth, and nqr expression is modulated by electron acceptors and values of their redox potentials. The latter effect was shown to be independent of the ArcAB regulatory system.

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Abbreviations

Ap:

Ampicillin

CCCP:

Carbonyl cyanide m-chlorophenylhydrazone

DMSO:

Dimethyl sulfoxide

dNADH:

Reduced nicotinamide hypoxanthine dinucleotide

Km:

Kanamycin

Na+-NQR:

Na+-translocating NADH:quinone oxidoreductase

NDH-1:

H+-translocating NADH:quinone oxidoreductase

NDH-2:

Non-coupled NADH:quinone oxidoreductase

NpGal:

o-nitrophenyl-β-d-galactopyranoside

PCR:

Polymerase chain reaction

SBP:

Sub-bacterial particles

Rf:

Rifampicin

Tc:

Tetracycline

TMAO:

Trimethylamine N-oxide

\(\Delta \mu _{{\text{Na}^{+}}} \) :

Transmembrane difference in electrochemical potentials of sodium ions.

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Acknowledgments

This work was supported in part by the Russian Foundation for Basic Research (grant 07-04-00619). The authors are grateful to the Genome Sequencing Center at Washington University Medical School for communicating the V. harveyi and K. pneumoniae DNA sequence data prior to publication.

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Author notes

  1. Galina A. Belevich

    Present address: Institute of Biotechnology, University of Helsinki, P.O. Box 65, Viikinkaari 1, 00014, Helsinki, Finland

Authors and Affiliations

  1. Department of Molecular Energetics of Microorganisms, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory, Moscow, 119992, Russia

    Maria S. Fadeeva, Evgenia A. Yakovtseva, Galina A. Belevich, Yulia V. Bertsova & Alexander V. Bogachev

Authors
  1. Maria S. Fadeeva
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  2. Evgenia A. Yakovtseva
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  3. Galina A. Belevich
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  4. Yulia V. Bertsova
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  5. Alexander V. Bogachev
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Corresponding author

Correspondence to Alexander V. Bogachev.

Additional information

Communicated by David Kelly.

Accession number: EF394942 (Vibrio harveyi arcB gene, partial cds).

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203_2007_254_MOESM1_ESM.doc

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Fadeeva, M.S., Yakovtseva, E.A., Belevich, G.A. et al. Regulation of expression of Na+-translocating NADH:quinone oxidoreductase genes in Vibrio harveyi and Klebsiella pneumoniae . Arch Microbiol 188, 341–348 (2007). https://doi.org/10.1007/s00203-007-0254-5

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  • DOI: https://doi.org/10.1007/s00203-007-0254-5

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