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Plasma membrane of Synechocystis PCC 6803: a heterogeneous distribution of membrane proteins

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Abstract

Proteomic studies carried out previously on the plasma membrane of Synechocystis have identified several peripheral and integral proteins. The distribution of these proteins along the membrane still remains obscure. In this study, the distribution of proteins along the plasma membrane of Synechocystis was carried out using subfractions, the right-side-out (RSO) and inside-out (ISO) vesicles, fractionated from a pure and specific fraction of the plasma membrane. These subfractions were analyzed and quantified for several proteins by immunoblotting. It was found that the ISO fraction contained higher quantities of preD1, D1 and PsaD, the integral proteins of photosystem I and II known to be present also in the plasma membrane. Lower amounts of peripheral vesicle inducing protein Vipp1 and nitrate/nitrite binding protein NrtA were present in the ISO compared to the RSO fraction. On the contrary, the distribution of two integral transporter proteins, SbtA and PxcA, was found equal in both fractions. Our studies clearly establish that the plasma membrane of Synechocystis has a heterogeneous composition with respect to protein distribution. The accumulation of photosynthesis-associated proteins in the ISO fraction provides evidence that the discrete regions of the plasma membrane harbor sites for biogenesis of photosystems.

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Acknowledgement

The authors thank Virpi Paakkarinen for technical assistance. The antibodies to SbtA, PxcA and NrtA were generous gifts from Prof. T. Ogawa, Japan. The Vipp1, preD1 and PsaD antibodies were kindly provided by Dr. UC Vothknecht, Germany, Prof. N. Murata, Japan and Dr. P. Chitnis, USA, respectively. This work was financially supported by the Academy of Finland (E.-M. A.) and the Carl Trygger Foundation (B.N.).

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  1. Renu Srivastava

    Present address: Plant Science Institute, Roy J Carver Co-Laboratory, Iowa State University, Ames, IA, 50010-3650, USA

Authors and Affiliations

  1. Department of Biology, Plant Physiology and Molecular Biology, University of Turku, 20014, Turku, Finland

    Renu Srivastava, Natalia Battchikova & Eva-Mari Aro

  2. Department of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm University, 10691, Stockholm, Sweden

    Birgitta Norling

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  1. Renu Srivastava
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  2. Natalia Battchikova
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  3. Birgitta Norling
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  4. Eva-Mari Aro
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Correspondence to Eva-Mari Aro.

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Srivastava, R., Battchikova, N., Norling, B. et al. Plasma membrane of Synechocystis PCC 6803: a heterogeneous distribution of membrane proteins. Arch Microbiol 185, 238–243 (2006). https://doi.org/10.1007/s00203-006-0086-8

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  • DOI: https://doi.org/10.1007/s00203-006-0086-8

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