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Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system

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Abstract

The tricarboxylic acid (TCA) cycle is one of the major routes of carbon catabolism in Bacillus subtilis. The syntheses of the enzymes performing the initial reactions of the cycle, citrate synthase, and aconitase, are synergistically repressed by rapidly metabolizable carbon sources and glutamine. This regulation involves the general transcription factor CcpA and the specific repressor CcpC. In this study, we analyzed the expression and intracellular localization of CcpC. The synthesis of citrate, the effector of CcpC, requires acetyl-CoA. This metabolite is located at a branching point in metabolism. It can be converted to acetate in overflow metabolism or to citrate. Manipulations of the fate of acetyl-CoA revealed that efficient citrate synthesis is required for the expression of the citB gene encoding aconitase and that control of the two pathways utilizing acetyl-CoA converges in the control of citrate synthesis for the induction of the TCA cycle. The citrate pool seems also to be controlled by arginine catabolism. The presence of arginine results in a severe CcpC-dependent repression of citB. In addition to regulators involved in sensing the carbon status of the cell, the pleiotropic nitrogen-related transcription factor, TnrA, activates citB transcription in the absence of glutamine.

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Abbreviations

TCA:

Tricarboxylic acid

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Acknowledgments

We are grateful to Sabine Lentes for her help with some of the experiments. Linc Sonenshein is acknowledged for the critical reading of the manuscript. This work was supported by grants from the Deutsche Forschungsgemeinschaft (Stu 214/2-1; Stu 214/2-2) and the Fonds der Chemischen Industrie.

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  1. Abteilung für Allgemeine Mikrobiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany

    Hans-Matti Blencke, Irene Reif, Fabian M. Commichau & Jörg Stülke

  2. Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität, Erlangen-Nürnberg, Germany

    Hans-Matti Blencke, Irene Reif, Christian Detsch, Ingrid Wacker, Holger Ludwig & Jörg Stülke

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  1. Hans-Matti Blencke
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  2. Irene Reif
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  3. Fabian M. Commichau
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  4. Christian Detsch
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Correspondence to Jörg Stülke.

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Blencke, HM., Reif, I., Commichau, F.M. et al. Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system. Arch Microbiol 185, 136–146 (2006). https://doi.org/10.1007/s00203-005-0078-0

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  • DOI: https://doi.org/10.1007/s00203-005-0078-0

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