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Expression of the UGA4 gene encoding the δ-aminolevulinic and γ-aminobutyric acids permease in Saccharomyces cerevisiae is controlled by amino acid-sensing systems

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Abstract

In yeasts, several sensing systems localized to the plasma membrane which transduce information regarding the availability and quality of nitrogen and carbon sources and work in parallel with the intracellular nutrient-sensing systems, regulate the expression and activity of proteins involved in nutrient uptake and utilization. The aim of this work was to establish whether the cellular signals triggered by amino acids modify the expression of the UGA4 gene which encodes the δ-aminolevulinic (ALA) and γ-aminobutyric (GABA) acids permease. In the present paper, we demonstrate that extracellular amino acids regulate UGA4 expression and that this effect seems to be mediated by the amino acid sensor complex SPS (SSY1, PTR3, SSY5).

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Acknowledgements

This work was supported by grants from the Argentine National Research Council (CONICET) (PEI6306-2004), the UBACYT X195 (2001–2003) and the UBACYT X014 (2004–2007). Mariana Bermúdez Moretti and Susana Correa García hold the post of Associate Researchers at the CONICET. Alcira Batlle holds the post of Superior Researcher at the CONICET.

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Authors and Affiliations

  1. Departamento de Química Biológica (FCEyN, UBA), Ciudad Universitaria, Pabellón II, 4° Piso, 1428, Buenos Aires, Argentina

    Mariana Bermudez Moretti & Susana Correa Garcia

  2. Departamento de Fisiología y Biología Molecular y Celular (FCEyN, UBA), Ciudad Universitaria, Pabellón II, 2° Piso, 1428, Buenos Aires, Argentina

    Mariana Bermudez Moretti & Susana Correa Garcia

  3. Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET, Depto. Química Biológica (FCEyN, UBA), Ciudad Universitaria, Pabellón II, 2° Piso, 1428, Buenos Aires, Argentina

    Ana Mercedes Perullini & Alcira Batlle

  4.  , Rivadavia 1583 9°B, 1033, Buenos Aires, Argentina

    Mariana Bermudez Moretti

Authors
  1. Mariana Bermudez Moretti
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  2. Ana Mercedes Perullini
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  3. Alcira Batlle
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  4. Susana Correa Garcia
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Correspondence to Mariana Bermudez Moretti.

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Bermudez Moretti, M., Perullini, A.M., Batlle, A. et al. Expression of the UGA4 gene encoding the δ-aminolevulinic and γ-aminobutyric acids permease in Saccharomyces cerevisiae is controlled by amino acid-sensing systems. Arch Microbiol 184, 137–140 (2005). https://doi.org/10.1007/s00203-005-0022-3

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  • DOI: https://doi.org/10.1007/s00203-005-0022-3

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