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Mass spectrometry proteomic analysis of stress adaptation reveals both common and distinct response pathways in Propionibacterium freudenreichii

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Abstract

Microorganisms used in food technology and probiotics are exposed to technological and digestive stresses, respectively. Traditionally used as Swiss-type cheese starters, propionibacteria also constitute promising human probiotics. Stress tolerance and cross-protection in Propionibacterium freudenreichii were thus examined after exposure to heat, acid, or bile salts stresses. Adapted cells demonstrated acquired homologous tolerance. Cross-protection between bile salts and heat adaptation was demonstrated. By contrast, bile salts pretreatment sensitized cells to acid challenge and vice versa. Surprisingly, heat and acid responses did not present significant cross-protection in P. freudenreichii. During adaptations, important changes in cellular protein synthesis were observed using two-dimensional electrophoresis. While global protein synthesis decreased, several proteins were overexpressed during stress adaptations. Thirty-four proteins were induced by acid pretreatment, 34 by bile salts pretreatment, and 26 by heat pretreatment. Six proteins are common to all stresses and represent general stress-response components. Among these polypeptides, general stress chaperones, and proteins involved in energetic metabolism, oxidative stress response, or SOS response were identified. These results bring new insight into the tolerance of P. freudenreichii to heat, acid, and bile salts, and should be taken into consideration in the development of probiotic preparations.

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Acknowledgements

Standa Industrie is greatly acknowledged for financial support and for constant interest and enthusiasm in this work. P. Leverrier is the recipient of a grant from the Institut National de la Recherche Agronomique and from Standa Industrie. John Hannon is acknowledged for English correction.

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  1. Patrick Boyaval

    Present address: Rhodia Food, ZA de Buxières, BP 10, 86220, Dangé St Romain, France

Authors and Affiliations

  1. Laboratoire de Recherches de Technologie Laitière, Institut National de la Recherche Agronomique, 65 rue de St Brieuc, 35042, Rennes cedex, France

    Pauline Leverrier, Annette Rouault, Patrick Boyaval & Gwénaël Jan

  2. Standa Industrie, 68 rue R. Kaskoreff, 14050, Caen cedex 4, France

    Pauline Leverrier

  3. Waters Corporation-EU Mass Spectrometry Technologies Centre, Transistorstraat 18, 1322, CE Almere, The Netherlands

    Johannes P. C. Vissers

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Correspondence to Gwénaël Jan.

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Leverrier, P., Vissers, J.P.C., Rouault, A. et al. Mass spectrometry proteomic analysis of stress adaptation reveals both common and distinct response pathways in Propionibacterium freudenreichii . Arch Microbiol 181, 215–230 (2004). https://doi.org/10.1007/s00203-003-0646-0

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  • DOI: https://doi.org/10.1007/s00203-003-0646-0

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