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Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily

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Abstract

ORF MJ1605, previously annotated as pgi and coding for the putative glucose-6-phosphate isomerase (phosphoglucose isomerase, PGI) of the hyperthermophilic archaeon Methanococcus jannaschii, was cloned and functionally expressed in Escherichia coli. The purified 80-kDa protein consisted of a single subunit of 45 kDa, indicating a homodimeric (α2) structure. The K m values for fructose 6-phosphate and glucose 6-phosphate were 0.04 mM and 1 mM, the corresponding V max values were 20 U/mg and 9 U/mg, respectively (at 50 °C). The enzyme had a temperature optimum at 89 °C and showed significant thermostability up to 95 °C. The enzyme was inhibited by 6-phosphogluconate and erythrose-4-phosphate. RT-PCR experiments demonstrated in vivo expression of ORF MJ1618 during lithoautotrophic growth of M. jannaschii on H2/CO2. Phylogenetic analyses indicated that M. jannaschii PGI was obtained from bacteria, presumably from the hyperthermophile Thermotoga maritima.

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Acknowledgements

This work supported by the Fonds der Chemischen Industrie.

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  1. Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität Kiel, Am Botanischen Garten 1–9, 24118, Kiel, Germany

    Bente Rudolph, Thomas Hansen & Peter Schönheit

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  1. Bente Rudolph
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  2. Thomas Hansen
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  3. Peter Schönheit
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Correspondence to Peter Schönheit.

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Rudolph, B., Hansen, T. & Schönheit, P. Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily. Arch Microbiol 181, 82–87 (2004). https://doi.org/10.1007/s00203-003-0626-4

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  • DOI: https://doi.org/10.1007/s00203-003-0626-4

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