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Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B

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Abstract

A respiratory-deficient mutant of Candida albicans MEN was generated by culturing cells in medium supplemented with ethidium bromide at 37 °C for 5 days. The respiratory-deficient mutant (C. albicans MMU11) was incapable of growth on glycerol, had a reduced oxygen uptake rate and demonstrated an altered mitochondrial cytochrome profile. Respiratory-competent cybrids were formed by mitochondrial transfer following fusion of protoplasts with those of C. albicans ATCC 44990. Mutant MMU11 possessed lower levels of ergosterol than the parental isolates and the cybrids, and demonstrated a small but statistically significant increase in tolerance to amphotericin B. The results demonstrated that disruption of mitochondrial function in C. albicans increases the tolerance to amphotericin B, possibly mediated by a reduction in cellular ergosterol content.

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Acknowledgements

The authors are grateful to Bernie Coleman and Donal O'Shea for assistance with GC analysis. Patrick Geraghty is the recipient of a grant from the Irish American Partnership. This work was supported by funding to the National Institute for Cellular Biotechnology under PRTLI 3.

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  1. Medical Myology Unit, National Institute for Cellular Biotechnology, National University of Ireland Maynooth, Department of Biology, Co. Kildare, Ireland

    Patrick Geraghty & Kevin Kavanagh

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  1. Patrick Geraghty
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  2. Kevin Kavanagh
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Correspondence to Kevin Kavanagh.

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Geraghty, P., Kavanagh, K. Disruption of mitochondrial function in Candida albicans leads to reduced cellular ergosterol levels and elevated growth in the presence of amphotericin B. Arch Microbiol 179, 295–300 (2003). https://doi.org/10.1007/s00203-003-0530-y

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  • DOI: https://doi.org/10.1007/s00203-003-0530-y

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