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Effects of risedronate on trabecular microstructure and biomechanical properties in ovariectomized rat tibia

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Abstract

We determined the effect of risedronate on the trabecular microstructure of ovariectomized rat tibiae, using micro-computed tomography, in order to investigate how changes in microstructure contribute to biomechanical properties. Fifty 18-week-old rats underwent sham operation (n=10) or ovariectomy (OVX) (n=40). The OVX rats were further divided into four groups (n=10 for each group) and treated with risedronate at doses of 0, 0.1, 0.5 or 2.5 mg/kg for 9 months. OVX caused deterioration of three-dimensional trabecular microstructure, notably structure model index (SMI) and connectivity density, while treatment of OVX rats with risedronate at 0.5 and 2.5 mg/kg improved those deleterious microstructural changes. Biomechanical property, as assessed by finite element analysis (FEA), correlated significantly with trabecular bone volume fraction (BV/TV), and the correlation further increased substantially when microstructural parameters were added, especially SMI and connectivity density, with risedronate therapy. Thus, it is suggested that, in addition to increasing bone mass, risedronate improves biomechanical property by maintaining a plate-like structure as well as connectivity of trabeculae.

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Acknowledgements

We thank Dr. Kyoji Ikeda (Department of Bone and Joint Disease, The Research Institute, National Center for Geriatrics and Gerontology) for suggestions on the manuscript. We are grateful for the assistance of Mr. Jun Kono (Department of Radiology, Nagasaki Saiseikai Hospital). This study was supported in part by the Program for Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research of Japan [Masako Ito (no. MF-14)] and by grant-in-aid for scientific research from the Ministry of Education in Japan [Masako Ito (no. 13670949)].

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Authors and Affiliations

  1. Department of Radiology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan

    Masako Ito, Akifumi Nishida, Masataka Uetani & Kuniaki Hayashi

  2. Department of Public Health, Nagasaki University School of Medicine, Nagasaki, Japan

    Kiyoshi Aoyagi

  3. Drug Analysis & Pharmacokinetics Research Laboratories, Pharmaceutical Research Division, Takeda Chemical Industries Ltd., Osaka, Japan

    Masahiro Kawase

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  1. Masako Ito
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  2. Akifumi Nishida
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  3. Kiyoshi Aoyagi
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  4. Masataka Uetani
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  5. Kuniaki Hayashi
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  6. Masahiro Kawase
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Correspondence to Masako Ito.

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Ito, M., Nishida, A., Aoyagi, K. et al. Effects of risedronate on trabecular microstructure and biomechanical properties in ovariectomized rat tibia. Osteoporos Int 16, 1042–1048 (2005). https://doi.org/10.1007/s00198-004-1802-3

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  • DOI: https://doi.org/10.1007/s00198-004-1802-3

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