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Impact of birth in the presence and absence of simulated birth injury on vaginal glycosaminoglycan content

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Abstract

Introduction and hypothesis

This study aims to evaluate the effects of simulated birth trauma and vaginal and Cesarean delivery on glycosaminoglycans (GAGs) in the vagina of female rats.

Methods

One hundred ten rats were divided into six groups: A (control), B (vaginal trauma), C (Cesarean delivery), D (Cesarean delivery followed by vaginal trauma), E (vaginal delivery), and F (20th day of gestation). In each group, half of the animals were killed 4 days after the procedure (time 1) and 12 weeks later (time 2). GAGs were extracted, isolated, and identified by using agarose gel electrophoresis and quantified by densitometry. Statistical analysis was performed using Kruskal–Wallis and Mann–Whitney tests.

Results

We observed a significant decrease in total GAGs and dermatan sulfate (DS) at time 1. Evaluation at time 2 showed a significant increase in total GAGs, DS, and heparan sulfate.

Conclusions

Levels of sulfated GAGs in the rat vagina are affected by delivery and simulated birth trauma.

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Conflicts of interest

None.

Financial Disclaimer

The Sao Paulo State Research Support Foundation (FAPESP) financially supported this study.

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Authors and Affiliations

  1. Section of Urogynecology and Pelvic Surgery/Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil

    José Maria Ruano, Paulo Cezar Feldner Jr, Claudia Cristina Takano, Rodrigo Aquino Castro, Marair Gracio Ferreira Sartori & Manoel João Batista Castello Girão

  2. Department of Biochemistry, Federal University of São Paulo, São Paulo, Brazil

    Helena Bonciani Nader

  3. Section of Urogynecology of Federal University of São Paulo, Rua dos Otonis, 601, 04025-001, São Paulo, SP, Brazil

    Paulo Cezar Feldner Jr

Authors
  1. José Maria Ruano
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  2. Paulo Cezar Feldner Jr
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  3. Claudia Cristina Takano
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  4. Rodrigo Aquino Castro
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  5. Helena Bonciani Nader
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  6. Marair Gracio Ferreira Sartori
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  7. Manoel João Batista Castello Girão
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Corresponding author

Correspondence to Paulo Cezar Feldner Jr.

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Ruano, J.M., Feldner, P.C., Takano, C.C. et al. Impact of birth in the presence and absence of simulated birth injury on vaginal glycosaminoglycan content. Int Urogynecol J 22, 1513–1519 (2011). https://doi.org/10.1007/s00192-011-1514-0

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  • DOI: https://doi.org/10.1007/s00192-011-1514-0

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