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Differential profile analysis of urinary cytokines in patients with overactive bladder

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International Urogynecology Journal Aims and scope Submit manuscript

An Erratum to this article was published on 27 May 2011

Abstract

Introduction and hypothesis

We hypothesize that overactive bladder (OAB) can produce inflammatory cytokines due to afferent neural plasticity or urothelial dysfunction. This study aimed to detect abnormal cytokine levels in urine of patients with OAB compared to urinary tract infections (UTI) and controls.

Methods

This was a prospective, single blind study including 20 premenopausal women (control), 20 with OAB and 16 with UTI. Urine samples were collected, centrifuged, and stored (−80°C). Urinary total proteins were quantified and detected by antibody-based array chip for release of 120 human cytokines in the two groups relative to the controls.

Results

Majority of cytokines showed the same expression in the OAB compared with the controls. Cytokines exclusively expressed in OAB were: monocyte chemoattractant protein (MCP) 1, TARC, PARC, and Fas/TNFRSF6. MCP-2, MCP-3, tumor necrosis factor-β, GCSF and eotaxin-3 showed a shared expression in UTI and OAB. Conversely, few of the cytokines were downregulated in OAB (IL-5, IL-6, IL-7, and GM-CSF).

Conclusions

Taken together, the results suggest that a subset of inflammatory cytokines and chemokines provides a framework for development of highly optimized urinary biomarker assay for differential diagnosis and treatment of OAB.

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Acknowledgment

Nuzhat Faruqui was funded by an IUGA International Fellowship Grant.

Conflicts of interest

None.

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Authors and Affiliations

  1. Section of Voiding Dysfunction and Female Urology, Cleveland Clinic Florida, Weston, FL, USA

    Gamal Ghoniem, Nuzhat Faruqui, Mostafa Elmissiry, Ayman Mahdy & Hassan Abdelwahab

  2. Department of Urology, Tulane University Health Sciences Center, New Orleans, LA, 70112, USA

    Mathew Oommen & Asim B. Abdel-Mageed

  3. Female Urology, Voiding Dysfunction and Pelvic Reconstructive Surgery, Cleveland Clinic Florida, 2950 Cleveland Clinic Blvd., Weston, FL, 33331, USA

    Gamal Ghoniem

Authors
  1. Gamal Ghoniem
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  2. Nuzhat Faruqui
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  4. Ayman Mahdy
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  5. Hassan Abdelwahab
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  6. Mathew Oommen
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  7. Asim B. Abdel-Mageed
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Corresponding author

Correspondence to Gamal Ghoniem.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00192-011-1471-7

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Ghoniem, G., Faruqui, N., Elmissiry, M. et al. Differential profile analysis of urinary cytokines in patients with overactive bladder. Int Urogynecol J 22, 953–961 (2011). https://doi.org/10.1007/s00192-011-1401-8

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  • DOI: https://doi.org/10.1007/s00192-011-1401-8

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