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Proteomic analysis of pubocervical fascia in women with and without pelvic organ prolapse and urodynamic stress incontinence

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Abstract

Introduction and hypothesis

This pilot study compares protein expression patterns in the pubocervical fascia of women with pelvic organ prolapse (POP) and stress urinary incontinence (SUI) and asymptomatic women with normal pelvic support.

Methods

Samples of pubocervical fascia were collected from four women with POP and SUI and from three asymptomatic (control) women. These were analyzed using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Results

The expression levels of transgelin, smooth muscle gamma-actin, myosin light polypeptide 6, and alpha-1 antitrypsin precursor were more than twofold higher in patients than in controls. An additional five proteins were overexpressed (more than twofold) in patients, while three proteins were detected only in the patients.

Conclusion

This pilot study is the first study to estimate changes in protein expression in the pubocervical fascia of human patients with POP. These changes could be related to the pathophysiology of POP.

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Abbreviations

POP:

Pelvic organ prolapse

UI:

Urinary incontinence

MS:

Mass spectrometry

2-DE:

Two-dimensional electrophoresis

MALDI-TOF-MS:

Matrix-assisted laser desorption/ionization time-of-flight MS

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Authors and Affiliations

  1. First Department of Obstetrics and Gynecology, Athens University School of Medicine, “Alexandra” Hospital, Vas Sofias 54, Athens, 11528, Greece

    Stavros Athanasiou, Elias Lymberopoulos, Alexandros Rodolakis, George Vlachos & Aris Antsaklis

  2. Biomedical Research Foundation, Academy of Athens, Athens, Greece

    Sofia Kanellopoulou

Authors
  1. Stavros Athanasiou
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  2. Elias Lymberopoulos
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  3. Sofia Kanellopoulou
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  4. Alexandros Rodolakis
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  5. George Vlachos
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  6. Aris Antsaklis
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Corresponding author

Correspondence to Stavros Athanasiou.

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Athanasiou, S., Lymberopoulos, E., Kanellopoulou, S. et al. Proteomic analysis of pubocervical fascia in women with and without pelvic organ prolapse and urodynamic stress incontinence. Int Urogynecol J 21, 1377–1384 (2010). https://doi.org/10.1007/s00192-010-1203-4

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  • DOI: https://doi.org/10.1007/s00192-010-1203-4

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