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LOX family enzymes expression in vaginal tissue of premenopausal women with severe pelvic organ prolapse

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Abstract

Introduction and Hypothesis

The extracellular matrix proteins collagen and elastin provide tissue strength and resilience, whereas lysyl oxidase enzymes play a major role in their stabilization. This study examines the expression and tissue localization of lysyl oxidase family proteins in the anterior vaginal wall of premenopausal women with advanced pelvic organ prolapse (POP, n = 15) and asymptomatic controls (n = 11). All women were in the proliferative phase of menstrual cycle.

Methods

Total mRNAs and proteins extracted from the vaginal tissue were examined by real-time polymerase chain reaction and immunoblotting, and tissue specimens were analyzed by immunohistochemistry.

Results

The expression of LOX, LOXL1, and LOXL3 genes as well as LOX and LOXL3 proteins were significantly reduced in POP patients (P < 0.05). Immunolocalization of LOX family proteins was confirmed in all vaginal specimens.

Conclusion

We proposed that reduced expression of LOX enzymes may result in defective assembly of pelvic tissues and development of POP.

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Acknowledgements

The study funding was provided by the Research Fund, Department of Obstetrics and Gynecology, Mount Sinai Hospital, Toronto. There is no conflict of interest to be disclosed.

We thank Dr. V. Miranda for assisting us in the statistical analysis.

Conflicts of interest

None.

Author information

Authors and Affiliations

  1. Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, Mount Sinai Hospital, University of Toronto, 700 University Avenue, Suite 3083, Toronto, ON, M5G 1Z5, Canada

    May Alarab, Maria AT Bortolini & Harold Drutz

  2. Department of Obstetrics and Gynecology, University of Toronto, Toronto, M5S 1A1, Canada

    May Alarab, Maria AT Bortolini, Harold Drutz & Stephen Lye

  3. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, Canada

    May Alarab, Stephen Lye & Oksana Shynlova

Authors
  1. May Alarab
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  2. Maria AT Bortolini
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  3. Harold Drutz
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  4. Stephen Lye
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  5. Oksana Shynlova
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Corresponding author

Correspondence to May Alarab.

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Supplemental Fig. 1

Double immunostaining for CD68 and LOXL1 proteins. Immunofluorescence microscopy stained with antibodies against LOXL1 (C, red color), CD68 (B, green color), and nuclear staining (A, blue color). Coexpression of LOXL1 and CD68 by the same cell is indicated by yellow color (overlap of green and red). Magnification is ×400; scale bar = 25 μm (GIF 22 kb)

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Alarab, M., Bortolini, M.A., Drutz, H. et al. LOX family enzymes expression in vaginal tissue of premenopausal women with severe pelvic organ prolapse. Int Urogynecol J 21, 1397–1404 (2010). https://doi.org/10.1007/s00192-010-1199-9

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  • DOI: https://doi.org/10.1007/s00192-010-1199-9

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