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Hamstring autografts are associated with a high rate of contamination in anterior cruciate ligament reconstruction

  • Knee
  • Published:
Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

Purpose

To quantitatively evaluate the rate, type, and level of contamination of anterior cruciate ligament (ACL) hamstring autografts after harvesting and preparation or dropping onto the operating room (OR) floor.

Methods

Two hamstring autograft specimens were prospectively retrieved from each graft in a consecutive series of 50 patients undergoing primary isolated ACL reconstruction (100 specimens total). One specimen was retrieved immediately after harvesting and dropped onto the OR floor (dropped group). The other was retrieved just after graft implantation and before fixation (control group). Each specimen was incubated for aerobic and anaerobic growth, and the number of colony-forming units (CFU)/g was measured. Patients’ clinical course was monitored for signs of surgical site infection (SSI).

Results

The control and dropped groups had positive culture rates of 11/50 (22%) and 16/50 (32%), respectively, with no significant difference between groups (n.s.). The most common organism in the control group was Staphylococcus epidermidis (45.5%) followed by S. aureus (36.4%). In the dropped group, the most common organism was S. epidermidis (31.3%) followed by Bacillus species (25%). The median (range) CFU/g among positive specimens in the dropped and control groups was 65 (8–150) and 10 (2–60), respectively (P = 0.0003). No patient developed postoperative SSI.

Conclusion

Intraoperative hamstring autograft contamination rates were high. Hence, routine prophylactic decontamination of all hamstring autografts after harvesting and preparation and before implantation is recommended.

Level of evidence

Controlled laboratory study.

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Acknowledgements

The authors would like to thank College of Medicine Research Center, Deanship of Scientific Research at King Saud University for supporting our project.

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Authors and Affiliations

  1. Head of Sports Medicine and Arthroscopy Division, Orthopaedic Department, College of Medicine, King Saud University, Riyadh, Saudi Arabia

    Abdulaziz Z. Alomar

  2. Orthopaedic Department, College of Medicine, King Saud University, Riyadh, Saudi Arabia

    Saud M. Alfayez

  3. Department of Pathology and Laboratory Medicine/Microbiology Unit, College of Medicine, King Saud University, Riyadh, Saudi Arabia

    Ali M. Somily

Authors
  1. Abdulaziz Z. Alomar
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  2. Saud M. Alfayez
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  3. Ali M. Somily
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Correspondence to Abdulaziz Z. Alomar.

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The authors declared that they have no conflict of interests.

Funding

No funding was received for this study.

Ethical approval

The institutional review board of King Saud University granted approval for the study before it was conducted (No.:13/3824/IRB).

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Alomar, A.Z., Alfayez, S.M. & Somily, A.M. Hamstring autografts are associated with a high rate of contamination in anterior cruciate ligament reconstruction. Knee Surg Sports Traumatol Arthrosc 26, 1357–1361 (2018). https://doi.org/10.1007/s00167-017-4686-4

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  • DOI: https://doi.org/10.1007/s00167-017-4686-4

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