Skip to main content
Log in

The arrangement and the attachment areas of three ACL bundles

  • Knee
  • Published:
Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

Purpose

Normal anterior cruciate ligament (ACL) can be divided into three fiber bundles—i.e., anteromedial (AM), intermediate (IM), and posterolateral (PL) bundles. However, their arrangement and attachment areas had remained unclear. The purpose of this study was to clarify the arrangement of these three ACL fiber bundles and their attachment sites, and to provide information on the tunnel placement in anatomical triple bundle ACL reconstruction.

Methods

Seven non-embalmed human frozen knees were used. ACL fibers were bluntly divided into three bundles. A different-colored thread was wound around each fiber bundle in a spiral. Macroscopical investigation was performed to clarify the arrangement of three ACL bundles. Each fiber bundle was carefully detached from the femur and tibia, and then the distribution of attachment sites of each fiber bundle was observed.

Results

In knee extension, all bundles consisting of AM, IM and PL bundles ran parallel to each other in the lateral view from the medial side. The AM bundle overlapped with the IM bundle, whereas the PL bundle ran parallel to them on the distal aspects. As the knee flexion increased, the bundles became twisted around each other. On the tibial side, the attachment areas of three fiber bundles formed a triangular shape showing arrangements of AM, IM and PL bundles on the anteromedial, anterolateral and posterior aspects, respectively. On the femoral side, the PL bundle was attached on the distal-posterior areas; the IM bundle was attached distal-anterior to the AM bundle. They were arranged in a triangular shape on the tibia side as well.

Conclusion

This study clarified the arrangement of three fiber bundles of ACL and detailed geographical locations of their attachment sites. The detailed anatomic description of the natural ACL attachment might suggest to surgeons where to make tunnels during anatomical double/triple bundle ACL reconstruction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Amis AA, Dawkins GP (1991) Functional anatomy of the anterior cruciate ligament. Fibre bundle actions related to ligament replacements and injuries. J Bone Joint Surg Br 73:260–267

    PubMed  CAS  Google Scholar 

  2. Amis AA, Jakob RP (1998) Anterior cruciate ligament graft positioning, tensioning and twisting. Knee Surg Sports Traumatol Arthrosc 6(Suppl 1):S2–12

    Article  PubMed  Google Scholar 

  3. Arnoczky SP (1983) Anatomy of the anterior cruciate ligament. Clin Orthop Relat Res 172:19–25

    PubMed  Google Scholar 

  4. Benjamin M, Ralphs JR (2001) Entheses—the bony attachments of tendons and ligaments. Ital J Anat Embryol 106:151–157

    PubMed  CAS  Google Scholar 

  5. Colombet P, Robinson J, Christel P (2006) Morphology of anterior cruciate ligament attachments for anatomic reconstruction: a cadaveric dissection and radiographic study. Arthroscopy 22:984–992

    Article  PubMed  Google Scholar 

  6. Dargel J, Pohl P, Tzikaras P et al (2006) Morphometric side-toside differences in human cruciate ligament insertions. Surg Radiol Anat 28:398–402

    Article  PubMed  Google Scholar 

  7. Ferretti M, Ekdahl M, Shen W, Fu FH (2007) Osseous landmarks of the femoral attachment of the anterior cruciate ligament: an anatomic study. Arthroscopy 23:1218–1225

    Article  PubMed  Google Scholar 

  8. Friederich NF, O’Brien WR (1992) Clinical application of biomechanic and functional anatomical findings of the knee joint. Orthopade 21:41–50

    PubMed  CAS  Google Scholar 

  9. Fujie H, Otsubo H, Shino K et al (2011) Mechanical functions of the three bundles consisting of the human anterior cruciate ligament. Knee Surg Sports Traumatol Arthrosc PMID:21541712

  10. Georgoulis AD (2007) Tibial rotation is not restored after ACL reconstruction with a hamstring graft. Clini Orthop Relat Res 454:89–94

    Article  Google Scholar 

  11. Girgis FG, Marshall JL, Monajem A (1975) The cruciate ligaments of the knee joint. Anatomical, functional and experimental analysis. Clin Orthop Relat Res 106:216–231

    Article  PubMed  Google Scholar 

  12. Giron F, Cuomo P, Edwards A, Bull AM, Amis AA, Aglietti P (2007) Double-bundle “anatomic” anterior cruciate ligament reconstruction: a cadaveric study of tunnel positioning with a transtibial technique. Arthroscopy 23:7–13

    Article  PubMed  Google Scholar 

  13. Harner CD, Baek GH, Vogrin TM et al (1999) Quantitative analysis of human cruciate ligament insertions. Arthroscopy 15:741–749

    Article  PubMed  CAS  Google Scholar 

  14. Hamada M, Shino K, Horibe S, Mitsuoka T, Miyama T, Shiozaki Y, Mae T (2001) Single- versus bi-socket anterior cruciate ligament reconstruction using autogenous multiple-stranded hamstring tendons with endobutton femoral fixation: a prospective study. Arthroscopy 17:801–807

    PubMed  CAS  Google Scholar 

  15. Hart AJ, Buscombe J, Malone A, Dowd GS (2005) Assessment of osteoarthritis after reconstruction of the anterior cruciate ligament: a study using single-photon emission computed tomography at ten years. J Bone Joint Surg Br 87:1483–1487

    Article  PubMed  CAS  Google Scholar 

  16. Hutchinson MR, Ash SA (2003) Resident’s ridge: assessing the cortical thickness of the lateral wall and roof of the intercondylar notch. Arthroscopy 19:931–935

    Article  PubMed  Google Scholar 

  17. Iwahashi T, Shino K, Nakata K et al (2008) Assessment of the “functional length” of the three bundles of the anterior cruciate ligament. Knee Surg Sports Traumatol Arthrosc 16:167–174

    Article  PubMed  Google Scholar 

  18. Iwahashi T, Shino K, Otsubo H et al (2010) Direct anterior cruciate ligament insertion to the femur assessed by histology and 3-dimensional volume-rendered computed tomography. Arthroscopy 26:S13–S20

    Article  PubMed  Google Scholar 

  19. Kopf S, Musahl V, Tashman S, Szczodry M, Shen W, Fu FH (2008) A systematic review of the femoral origin and tibial insertion morphology of the ACL. Arthroscopy 17:213–219

    Google Scholar 

  20. Kondo E, Yasuda K, Azuma H, Tanabe Y, Yagi T (2008) Prospective clinical comparisons of anatomic double-bundle versus single-bundle anterior cruciate ligament reconstruction procedures in 328 consecutive patients. Am J Sports Med 36:1675–1687

    Article  PubMed  Google Scholar 

  21. Loh JC, Fukuda Y, Tsuda E, Steadman RJ, Fu FH, Woo SL (2003) Knee stability and graft function following anterior cruciate ligament reconstruction: comparison between 11 o’clock and 10 o’clock femoral tunnel placement. Arthroscopy 19:297–304

    Article  PubMed  Google Scholar 

  22. Luites JW, Wymenga AB, Blankevoort L et al (2007) Description of the attachment geometry of the anteromedial and posterolateral bundles of the ACL from arthroscopic perspective for anatomical tunnel placement. Knee Surg Sports Traumatol Arthrosc 15:1422–1431

    Article  PubMed  Google Scholar 

  23. Mae T, Shino K, Matsumoto N, Nakata K, Nakamura N, Iwahashi T (2006) Force sharing between two grafts in the anatomical two-bundle anterior cruciate ligament reconstruction. Knee Surg Sports Traumatol Arthrosc 14:505–509

    Article  PubMed  Google Scholar 

  24. Maeda A, Shino K, Horibe S, Nakata K, Buccafusca G (1996) Anterior cruciate ligament reconstruction with multistranded autogenous semitendinosus tendon. Am J Sports Med 24:504–509

    Article  PubMed  CAS  Google Scholar 

  25. Mochizuki T, Muneta T, Nagase T, Shirasawa S, Akita K, Sekiya I (2006) Cadaveric knee observation study for describing anatomic femoral tunnel placement for two-bundle anterior cruciate ligament reconstruction. Arthroscopy 22:356–361

    Article  PubMed  Google Scholar 

  26. Mommersteeg TJ, Kooloos JG, Roeling FQ et al (1995) The fibre bundle anatomy of human cruciate ligaments. J Anat 187:461–471

    PubMed  Google Scholar 

  27. Morimoto Y, Ferretti M, Ekdahl M, Smolinski P, Fu FH (2009) Tibiofemoral joint contact area and pressure after single- and double-bundle anterior cruciate ligament reconstruction. Arthroscopy 25:62–69

    Article  PubMed  Google Scholar 

  28. Muneta T, Takakuda K, Yamamoto H (1997) Intercondylar notch width and its relation to the configuration and cross-sectional area of the anterior cruciate ligament. A cadaveric knee study. Am J Sports Med 25:69–72

    Article  PubMed  CAS  Google Scholar 

  29. Musahl V, Voos JE, O’Loughlin PF, Choi D, Stueber V, Kendoff D, Pearle AD (2010) Comparing stability of different single- and double-bundle anterior cruciate ligament reconstruction techniques: a cadaveric study using navigation. Arthroscopy 26:S41–S48

    Article  PubMed  Google Scholar 

  30. Norwood LA, Cross MJ (1979) Anterior cruciate ligament: functional anatomy of its bundles in rotatory instabilities. Am J Sports Med 7:23–26

    Article  PubMed  CAS  Google Scholar 

  31. Odensten M, Gillquist J (1985) Functional anatomy of the anterior cruciate ligament and a rationale for reconstruction. J Bone Joint Surg Am 67:257–262

    PubMed  CAS  Google Scholar 

  32. Otsubo H, Shino K, Nakamura N, Nakata K, Nakagawa S, Koyanagi M (2007) Arthroscopic evaluation of ACL grafts reconstructed with the anatomical two-bundle technique using hamstring tendon autograft. Knee Surg Sports Traumatol Arthrosc 15:720–728

    Article  PubMed  Google Scholar 

  33. Paschos NK, Gartzonikas D, Barkoula NM, Moraiti C, Paipetis A, Matikas TE, Georgoulis AD (2010) Cadaveric study of anterior cruciate ligament failure patterns under uniaxial tension along the ligament. Arthroscopy 26:957–967

    Article  PubMed  Google Scholar 

  34. Pietrini SD, Ziegler CG, Anderson CJ, Wijdicks CA, Westerhaus BD, Johansen S, Engebretsen L, Laprade RF (2011) Radiographic landmarks for tunnel positioning in double-bundle ACL reconstructions. Knee Surg Sports Traumatol Arthrosc 19:792–800

    Article  PubMed  Google Scholar 

  35. Pombo MW, Shen W, Fu FH (2008) Anatomic double-bundle anterior cruciate ligament reconstruction: where are we today? Arthroscopy 24:1168–1177

    Article  PubMed  Google Scholar 

  36. Purnell ML, Larson AI, Clancy W (2008) Anterior cruciate ligament insertions on the tibia and femur and their relationships to critical bony landmarks using high-resolution volume-rendering computed tomography. Am J Sports Med 36:2083–2090

    Article  PubMed  Google Scholar 

  37. Shino K, Nakata K, Otsubo H et al (2005) Anatomic anterior cruciate ligament reconstruction using two double-looped hamstring tendon grafts via twin femoral and triple tibial tunnels. Oper Tech Orthop Surg 15:130–134

    Article  Google Scholar 

  38. Shino K, Nakata K, Nakamura N, Toritsuka Y, Nakagawa S, Horibe S (2005) Anatomically oriented anterior cruciate ligament reconstruction with a bone-patellar tendon-bone graft via rectangular socket and tunnel: a snug-fit and impingement-free grafting technique. Arthroscopy 21:1402

    PubMed  Google Scholar 

  39. Shino K, Nakata K, Nakamura N, Toritsuka Y, Horibe S, Nakagawa S, Suzuki T (2008) Rectangular tunnel double-bundle anterior cruciate ligament reconstruction with bone-patellar tendon-bone graft to mimic natural fiber arrangement. Arthroscopy 24:1178–1183

    Article  PubMed  Google Scholar 

  40. Shino K, Suzuki T, Iwahashi T (2010) The resident’s ridge as an arthroscopic landmark for anatomical femoral tunnel drilling in ACL reconstruction. Knee Surg Sports Traumatol Arthrosc 18:1164–1168

    Article  PubMed  Google Scholar 

  41. Siebold R, Ellert T, Metz S et al (2008) Femoral insertions of the anteromedial and posterolateral bundles of the anterior cruciate ligament: morphometry and arthroscopic orientation models for double-bundle bone tunnel placement—a cadaver study. Arthroscopy 24:585–592

    Article  PubMed  Google Scholar 

  42. Siebold R, Ellert T, Metz S et al (2008) Tibial insertions of the anteromedial and posterolateral bundles of the anterior cruciate ligament: morphometry, arthroscopic landmarks, and orientation model for bone tunnel placement. Arthroscopy 24:154–161

    Article  PubMed  Google Scholar 

  43. Takahashi M, Doi M, Abe M et al (2006) Anatomical study of the femoral and tibial insertions of the anteromedial and posterolateral bundles of human anterior cruciate ligament. Am J Sports Med 34:787–792

    Article  PubMed  Google Scholar 

  44. Toritsuka Y, Amano H, Kuwano M, Iwai T, Mae T, Ohzono K, Shino K (2009) Outcome of double-bundle ACL reconstruction using hamstring tendons. Knee Surg Sports Traumatol Arthrosc 17:456–463

    Article  PubMed  Google Scholar 

  45. Tsukada H, Ishibashi Y, Tsuda E, Fukuda A, Toh S (2008) Anatomical analysis of the anterior cruciate ligament femoral and tibial footprints. J Orthop Sci 13:122–129

    Article  PubMed  Google Scholar 

  46. Yamakami Y et al (1998) The accuracy of square measure of regions of interest using an NIH image. J Showa Univ Dent Soc 18:400–405

    Google Scholar 

  47. Zantop T, Wellmann M, Fu FH, Petersen W (2008) Tunnel positioning of anteromedial and posterolateral bundles in anatomic anterior cruciate ligament reconstruction: anatomic and radiographic findings. Am J Sports Med 36:65–72

    Article  PubMed  Google Scholar 

  48. Zhao J, Huangfu X (2010) Arbitrary starting point of separation affects morphology of the 2 bundles of anterior cruciate ligament at insertion sites. Arthroscopy 26:184–191

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hidenori Otsubo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Otsubo, H., Shino, K., Suzuki, D. et al. The arrangement and the attachment areas of three ACL bundles. Knee Surg Sports Traumatol Arthrosc 20, 127–134 (2012). https://doi.org/10.1007/s00167-011-1576-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00167-011-1576-z

Keywords

Navigation