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ACL reconstruction in a rabbit model using irradiated Achilles allograft seeded with mesenchymal stem cells or PDGF-B gene-transfected mesenchymal stem cells

  • Knee
  • Published:
Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

The present study was conducted to develop a new strategy to accelerate reconstruction of the anterior cruciate ligament (ACL) by modifying the Achilles allograft with autogenous mesenchymal stem cells (MSCs) or PDGF-B transfected MSCs in a rabbit model. The allografts were first irradiated with Co60, stored at −80°C, and then seeded with cells for implantation. Bilateral ACL reconstructions were performed. On the left, the allograft was either seeded with MSCs or PDGF-B transfected MSCs and acted as the experimental group. On the right, the graft without any cells seeded acted as control. At 3, 6 and 12 weeks after surgery, histological observation found that implatation of MSCs or PDGF-B transfected MSCs accelerated cellular infiltration into the ACL and enhanced collagen deposition in the wound. PDGF-B transfected MSCs could also lead to an initial promotion of angiogenesis. This gene transfer technique or cell implantion may be a potentially useful tool for improving ligament remodeling.

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Acknowledgments

We would like to thank Professor Carl-Henrik Heldin, Ludwig Institute for Cancer Research, Sweden and Christer Betsholtz, Institute of Medical Biochemistry, University of Gothenburg, Sweden for helping us in our study.

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Authors and Affiliations

  1. Institute of Sports Medicine, Peking University Third Hospital, No. 49, North Garden Road, Haidian District, Beijing, 100083, People’s Republic of China

    Feng Li & Changlong Yu

  2. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100083, People’s Republic of China

    Hongti Jia

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  1. Feng Li
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  2. Hongti Jia
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Correspondence to Changlong Yu.

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Li, F., Jia, H. & Yu, C. ACL reconstruction in a rabbit model using irradiated Achilles allograft seeded with mesenchymal stem cells or PDGF-B gene-transfected mesenchymal stem cells. Knee Surg Sports Traumatol Arthr 15, 1219–1227 (2007). https://doi.org/10.1007/s00167-007-0385-x

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