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Thiopental inhibits migration of human leukocytes through human endothelial cell monolayers in vitro

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Abstract

Objectives

The interactions between blood and vascular wall cells are essential for the understanding of pathophysiologic processes, e. g. inflammation. The influence of the anesthetic drug thiopental on leukocyte function is well documented. Recently, an inhibitory effect of thiopental on leukocyte chemotaxis in a Boyden chamber assay (i. e. endothelial cells were not included) was demonstrated. In vivo, leukocytes have to interact with endothelial cell monolayers to invade the tissue. The influence of thiopental on a monolayer of endothelial cells has not yet been investigated. The aim of the current study was to investigate the influence of thiopental on the migration of leukocytes through endothelial cell monolayers (ECM).

Material and methods

Human umbilical vein endothelial cells (HUVEC) were isolated and cultured on microporous membrane filters to achieve a monolayer. Isolated polymorphonuclear leukocytes (PMNL) as well as ECM were preincubated with different concentrations of thiopental. The rate of leukocyte migration against the chemotactic protein formyl-methyl-leucyl-phenyla-lanine was measured (n=7). Thiopental was able to reduce the amount of leukocyte migration through ECM significantly.

Conclusion

In conclusion, we could show that thiopental is able to reduce the migration of PMNL through ECM significantly.

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References

  1. Osborn L (1990) Leukocyte adhesion to endothelium during inflammation. Cell 62: 3–6

    Article  PubMed  CAS  Google Scholar 

  2. Carlos TM, Harlan JM (1994) Leukocyte-endothelial adhesion molecules. Blood 84: 2068–2101

    PubMed  CAS  Google Scholar 

  3. Issekutz AC, Chuluyan HE, Lopes N (1995) CD11/CD18-independent trans-endothelial migration of human polymorphonuclear leukocytes and monocytes: involvement of distinct and unique mechanism. J Leukoc Biol 57:55–61

    Google Scholar 

  4. Zimmermann GA, Prescott SM, Mclntyre TM (1992) Endothelial cell interactions with granulocytes: tethering and signaling molecules. Immunol Today 14: 93–100

    Article  Google Scholar 

  5. Skoutelis A, Lianou P, Papageorgiou E, Kokkinis K, Alexopoulos K, Bassaris H (1992) Effects of propofol and thiopental on polymorphonuclear leukocyte functions in vitro. Acta Anesthesiol Scand 38: 858–862

    Article  Google Scholar 

  6. Kress HG, Segmueller R (1987) Intravenous anesthetics and human neutrophil granulocyte motility in vitro. Anaesthesist 36: 356–361

    PubMed  CAS  Google Scholar 

  7. Kress HG, Eberlein T, Horber B, Weiss KH (1989) Suppression of neutrophil migration and chemiluminescence is due to the sulfur atom in the thiobarbiturate molecule. Acta Anesthesiol Scand 33:122–125

    Article  CAS  Google Scholar 

  8. Rossano F, Tufano R, Cipollaro-de-L’Ero G, Servillio G, Baroni G (1992) Anesthetic agents induce human mononuclear leukocytes to release cytokines. Immunopharmacol Immunotoxicol 14: 439–450

    Article  PubMed  CAS  Google Scholar 

  9. Mackay CR, Imhof BA (1993) Cell adhesion in the immune system. Immunol Today 14:111–114

    Article  Google Scholar 

  10. Nishina K, Akamatsu H, Mikawa K, Shiga M, Maskawa N, Hidefumi O, Niwa Y (1998) The inhibitory effects of thiopental, midazolam, and ketamine on human neutrophil functions. Anesth Analg 86:159–165

    Article  PubMed  CAS  Google Scholar 

  11. Fischer S, Renz D, Schaper W, Karliczek GF (1995) In vitro effects of fentanyl, methohexital and thiopental on brain endothelial cell permeability. Anesthesiol 82:451–458

    Article  CAS  Google Scholar 

  12. Jaffe EA, Nachman RL, Becker CG, Minick CR (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphological and immunological criteria. J Clin Invest 52:2745–2756

    Article  PubMed  CAS  Google Scholar 

  13. Shasby DM, Shasby SS (1990) Endothelial cells grown on filter membranes. In: Shasby DM and Shasby SS (eds) Cell Culture Techniques in Heart and Vessel Research. Springer-Verlag, New York, pp 86–121

    Google Scholar 

  14. Sunder-Plassmann G, Hofbauer R, Sengoelge G, Hoerl WH (1996) Quantification of leukocyte migration: improvement of a method. Immunol Invest 25: 49–63

    Article  PubMed  CAS  Google Scholar 

  15. Hofbauer R, Speiser W, Kapiotis S (1998) Ibuprofen inhibits leukocyte migration through endothelial cell monolayers. Life Science 62:1775–1781

    Article  CAS  Google Scholar 

  16. Weston SA, Parish CR (1990) New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J Immunol Methods 133: 87–97

    Article  PubMed  CAS  Google Scholar 

  17. Metcalf JA, Gallin JI, Nauseef WM, Root RK (1986) Preparation of cells and materials for functional assays. In: Metcalf JA (ed) Neutrophil purification. Raven Press, New York, p 5

    Google Scholar 

  18. Frohlich D, Rothe G, Schwall B, Schmitz G, Hobbhahn J, Taeger K (1996) Thiopentone and propofol, but not methohexitone or midazolam, inhibit neutrophil oxidative responses to the bacterial peptide FLMP. Eur J Anesth 13: 582–588

    Article  CAS  Google Scholar 

  19. Heine J, Leuwer M, Scheinichen D, Arseniev L, Jaeger K, Piepenbrock S (1996) Flow cytometry evaluation of the in vitro influence of four i. v. anaesthetics on respiratory burst of neutrophils. Brit J Anaesth 77: 387–392

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Anesthesiology and Intensive Care Medicine, University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria

    R. Hofbauer & H. Salfinger

  2. Clinical Institute of Medical and Chemical Laboratory Diagnostics, University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria

    R. Hofbauer & S. Kapiotis

  3. Department of Oral and Maxillofacial Surgery, University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria

    D. Moser

  4. Department of Internal Medicine I, Medical Intensive Care Unit 131.2, University of Vienna, Waehringer Guertel 18-20, A-1090, Vienna, Austria

    M. Frass

Authors
  1. R. Hofbauer
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  2. H. Salfinger
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  3. S. Kapiotis
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  4. D. Moser
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  5. M. Frass
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Correspondence to R. Hofbauer.

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Hofbauer, R., Salfinger, H., Kapiotis, S. et al. Thiopental inhibits migration of human leukocytes through human endothelial cell monolayers in vitro. Intensive Care Med 24, 973–976 (1998). https://doi.org/10.1007/s001340050698

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  • DOI: https://doi.org/10.1007/s001340050698

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