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Endothelial oxidative stress induced by serum from patients with severe trauma hemorrhage

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Abstract

Objective

Shock induces oxidative stress by ischemia-reperfusion phenomenon. Endothelial cells are involved in the inflammatory response and oxidative stress responsible for microcirculation impairment and organ failure. We examined the potential of serum from patients to induce in vitro reactive oxygen species production by cultured human umbilical vein endothelial cells (HUVECs).

Patients

Three groups were compared: hemorrhagic shock trauma patients, isolated brain injured patients, and healthy volunteers.

Methods

In the hemorrhagic shock group we sought a correlation between reactive oxygen species production and severity of shock. Serum was separated and perfused in an in vitro model of perfused HUVECs. Ex vivo reactive oxygen species production was assessed by fluorescence microscopy using dichlorodihydrofluorescein, an intracellular dye oxidized by H2O2. Results are expressed in proportional change from baseline and normalized by protidemia to control for variation related to hemodilution.

Results

Reactive oxygen species production by endothelial cells exposed to serum from hemorrhagic shock patients (46.2±24.9%) was significantly greater than in those with brain injury (3.9±35.1%) and in healthy volunteers (−6.8±5.8%). In the hemorrhagic shock group dichlorodihydrofluorescein fluorescence was strongly correlated positively to Simplified Acute Physiology Score II and lactatemia and negatively to [HCO3−].

Conclusions

Serum from trauma patients with hemorrhagic shock induces reactive oxygen species formation in naive endothelial cells which is correlated to shock severity.

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Authors and Affiliations

  1. Département d’Anesthésie-Réanimation Chirurgicale et UPRES EA 3540, Université Paris XI, Hôpital de Bicêtre, 78 Rue du Général Leclerc, 94270, le Kremlin Bicêtre, France

    Christian Laplace, Olivier Huet, Catherine Ract, Laurent Martin, Dan Benhamou & Jacques Duranteau

  2. Laboratoire d’Etude de la Microcirculation et Département de Biophysique, Université Paris VII, Hôpital Fernand Widal, Paris, France

    Eric Vicaut

Authors
  1. Christian Laplace
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  2. Olivier Huet
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  3. Eric Vicaut
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  4. Catherine Ract
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  5. Laurent Martin
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  6. Dan Benhamou
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  7. Jacques Duranteau
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Correspondence to Jacques Duranteau.

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Laplace, C., Huet, O., Vicaut, E. et al. Endothelial oxidative stress induced by serum from patients with severe trauma hemorrhage. Intensive Care Med 31, 1174–1180 (2005). https://doi.org/10.1007/s00134-005-2737-7

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  • DOI: https://doi.org/10.1007/s00134-005-2737-7

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