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Plasma oxidative parameters and mortality in patients with severe burn injury

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Abstract

Objective

To determine xanthine oxidase and superoxide dismutase activities, lipid peroxidation, protein carbonylation, and total radical-trapping antioxidant parameter in survivors and nonsurvivors patients with severe burn injury.

Design and setting

Prospective, comparative observational study in an intensive care unit, burn division, in a trauma hospital.

Patients

Twenty-five consecutive patients who met the established criteria for severe burn injury (total burn surface area of more than 30%).

Measurements and results

Plasma thiobarbituric acid reactive species and protein carbonyls levels were significantly higher in nonsurvivors than in survivors at 0 and 6 h. Elevated xanthine oxidase activity at 0 h was associated with adverse outcome after burn injury. In contrast, plasma superoxide dismutase activity and total radical-trapping antioxidant parameter did not differ significantly between nonsurvivors and survivors at any time point.

Conclusions

For the first time we demonstrate the value of oxidative parameters, namely thiobarbituric acid reactive species, protein carbonyls, and xanthine oxidase activity, in identifying burn patients with a poor prognosis. Whether these parameters are merely markers of clinical course, or whether they signal specific deleterious effects of oxidative stress during the burn injury remains to be elucidated.

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References

  1. LaLonde C, Knox J, Youn Y, Demling RH (1992) Relationship between hepatic blood flow and tissue liver lipid peroxidation in the early postburn period. Crit Care Med 20:789–796

    CAS  PubMed  Google Scholar 

  2. Saitoh D, Kadota T, Okada Y (1995) Direct evidence for the occurrence of superoxide radicals in the small intestine of the burned rat. Am J Emerg Med 13:37–40

    Article  CAS  PubMed  Google Scholar 

  3. Matsuda T, Taaka H, Williams S, Hanumadass M, Abcarian E, Reyes H (1991) Reduced fluid volume requirement for resuscitation of third-degree burns with high-dose vitamin C. J Burn Care Rehabil 12:525–532

    CAS  PubMed  Google Scholar 

  4. Horton JW, White J, Maass DL, Hybki DP, Haudek S, Giroir B (2001) Antioxidant vitamin therapy alters burn trauma-mediated cardiac NF-κB activation and cardiomyocyte cytokine secretion. J Trauma 50:397–408

    CAS  PubMed  Google Scholar 

  5. Klamt F, Dal-Pizzol F, Frota Jr MLC, Andrades M, Silva EG, Walz R, Izquierdo I, Brentani RR, Moreira JCF (2001) Imbalance of antioxidant defences in mice lacking cellular prion protein. Free Radic Biol Med 30:1137–1144

    Article  CAS  PubMed  Google Scholar 

  6. Tsai K, Hsu TG, Kong CW, Lin KC, Lu FG (2000) Is the endogenous peroxyl-radical scavenging capacity of plasma protective in systemic inflammatory disorders in humans? Free Radic Biol Med 28:926–933

  7. Bannister JV, Calaberese L (1987) Assays for SOD. Methods Biochem Anal 32:279–312

    CAS  PubMed  Google Scholar 

  8. Beckman JS, Parks DA, Marshall PA, Freeman BA (1989) A sensitive fluorometric assay for measuring xanthine dehydrogenase and oxidase in tissues. Free Radic Biol Med 6:607–615

    Article  CAS  PubMed  Google Scholar 

  9. Ribeiro CA, Andrade C, Polanczyk CA, Clausell N (2002) Association between early detection of soluble TNF-receptors and mortality in burn patients. Intensive Care Med 28:472–478

    Article  Google Scholar 

  10. Bertin-Maghit M, Goudable J, Dalmas E, Steghens JP, Bouchard C, Gueugniaud PY, Petit P, Delafosse B (2000) Time course of oxidative stress after major burns. Intensive Care Med 26:800–803

    CAS  PubMed  Google Scholar 

  11. Pintaudi AM, Tesoriere L, D'Arpa N, D'Amelio L, D'Arpa D, Bongiorno A, Masellis M, Livrea MA (2000) Oxidative stress after moderate to extensive burning in humans. Free Radic Res 33:139–146

    CAS  PubMed  Google Scholar 

  12. Németh I, Boda D (2001) Xanthine oxidase activity and blood glutathione redox ratio in infants and children with septic shock syndrome. Intensive Care Med 27:216–221

    Google Scholar 

  13. Saitoh D, Ookawara T, Fukuzuka K, Kawakami M, Sakamoto T, Ohno H, Okada Y (2001) Characteristics of plasma extracellular SOD in burned patients. Burns 27:577–581

    Article  CAS  PubMed  Google Scholar 

  14. Demling R, Ikegami K, Lalonde C (1995) Increased lipid peroxidation and decreased antioxidant activity correspond with death alter smoke exposure in the rat. J Burn Care Rehabil 16:104–110

    CAS  PubMed  Google Scholar 

  15. Farriol M, Fuentes F, Venereo Y, Solano I, Orta X, Segovia T (2001) Antioxidant capacity in severely burned patients. Pathol Biol (Paris) 49:227–31

    Google Scholar 

Download references

Acknowledgements

This work was supported by grants from CNPq, FAPERGS (Brazilian governmental agencies for research support), and the Hospital de Clínicas de Porto Alegre.

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Authors and Affiliations

  1. Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 anexo, 90035-003, Porto Alegre, RS, Brazil

    Cristiane Ritter, Michael Andrades, Márcio Guerreiro, Leonardo Zavaschi, Daniel Pens Gelain, Luis Fernando Souza, José Cláudio F. Moreira & Felipe Dal-Pizzol

  2. Centro de Pesquisa, Hospital de Clínicas, Porto Alegre, RS, Brazil

    Cristiane Ritter, Michael Andrades, Leonardo Zavaschi, Sérgio Menna-Barreto, José Cláudio F. Moreira & Felipe Dal-Pizzol

  3. Serviço de Medicina Intensiva, Hospital de Clínicas, Porto Alegre, RS, Brazil

    Cyntia A. Ribeiro

  4. Serviço de Cardiologia, Hospital de Clínicas, Porto Alegre, RS, Brazil

    Nadine Clausell

  5. Serviço de Pneumologia, Hospital de Clínicas, Porto Alegre,RS, Brazil

    Sérgio Menna-Barreto

  6. Departamento de Medicina, Universidade do Extremo Sul Catarinense, Porto Alegre, RS, Brazil

    Felipe Dal-Pizzol

Authors
  1. Cristiane Ritter
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  2. Michael Andrades
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  3. Márcio Guerreiro
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  4. Leonardo Zavaschi
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  5. Daniel Pens Gelain
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  6. Luis Fernando Souza
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  7. Cyntia A. Ribeiro
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  8. Nadine Clausell
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  9. Sérgio Menna-Barreto
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  10. José Cláudio F. Moreira
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  11. Felipe Dal-Pizzol
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Corresponding author

Correspondence to Felipe Dal-Pizzol.

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Ritter, C., Andrades, M., Guerreiro, M. et al. Plasma oxidative parameters and mortality in patients with severe burn injury. Intensive Care Med 29, 1380–1383 (2003). https://doi.org/10.1007/s00134-003-1833-9

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  • DOI: https://doi.org/10.1007/s00134-003-1833-9

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