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Microfluidic Paper Based Analytical Devices for the Detection of Carbamate Pesticides

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Abstract

Microfluidic paper-based analytical devices (μ-PADs) are a new technology platform for the development of extremely low-cost sensing applications. In this study, μ-PADs has been developed for quantitative determination of carbamate pesticides. Key experimental parameters including concentration and volume of acetylcholinesterase, acetylthiocholine iodide and 5,5′-dithiobis-(2-nitrobenzoic acid), incubation time and image capturing time were systematically optimized. Under optimal conditions, the method showed wide range of linearity (0.25–16 mg/L), repeatability (4%–5% RSD) and intermediate precision (7%–10% RSD). Limit of detection was observed to be 0.4, 0.24 and 0.46 mg/L for carbaryl, carbosulfan and furathiocarb, respectively. An acceptable mean recovery (87% to 94%) was observed for the three pesticides at 1 mg/L fortification level. The results reveal that the developed method requires minimal reagents, simple and is easy to handle. It can be used for the quantification of carbamate pesticides in resource limited laboratories without the need for the conventional analytical instruments.

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Acknowledgements

The authors would like to thank Addis Ababa University, Thematic Research Fund for supporting the project entitled “Developing Innovative Microfluidic Paper-Based Analytical Devices (μPADs): Viable solution for Environmental Monitoring in Ethiopia”. Sheleme also acknowledges North Shewa Zone Administration (Oromia) for sponsoring his PhD study.

Funding

This study was supported by Addis Ababa University (Grant No. VPRTT/PY-041/2018 to AH).

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Authors and Affiliations

  1. Center for Environmental Science, College of Natural and Computational Sciences, Addis Ababa University, P.O. Box:1176, Addis Ababa, Ethiopia

    Sheleme Beshana, Ahmed Hussen & Seyoum Leta

  2. Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University, Tsushimanana, Okayama, 700-8530, Japan

    Takashi Kaneta

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  1. Sheleme Beshana
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  2. Ahmed Hussen
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  3. Seyoum Leta
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  4. Takashi Kaneta
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Correspondence to Ahmed Hussen.

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Beshana, S., Hussen, A., Leta, S. et al. Microfluidic Paper Based Analytical Devices for the Detection of Carbamate Pesticides. Bull Environ Contam Toxicol 109, 344–351 (2022). https://doi.org/10.1007/s00128-022-03533-3

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  • DOI: https://doi.org/10.1007/s00128-022-03533-3

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