Abstract
Fish were exposed to a sub-lethal concentration of silver nanoparticles with and without orange and banana peels water treatment for 24, 48, and 96 h. The adsorption of AgNPs on both peels was recognized by scanning electron microscopic, energy dispersive X-ray spectroscopy, and laser ablation imaging. The % of DNA damage in liver and muscle tissues (comet assay) showed significant elevations in all studied groups with the maximum level in liver tissues after 24 h. DNA damage was markedly decreased after 48, and 96 h signifying the presence of an effective repairing mechanism. Micronucleus and nine nuclear anomalies were recorded in the peripheral blood cells. All anomalies were observed in all studied groups with a maximum induction rate after 96 h. of exposure. Based on the % of DNA damage and the frequencies of nuclear anomalies, water treatment with orange and banana peel succeeded to reduce AgNPs-induced genotoxic damage.
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Data Availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Abdel-Khalek AA (2015) Antioxidant responses and nuclear deformations in freshwater fish, Oreochromis niloticus, facing degraded environmental conditions. Bull Environ Contam Toxicol 94(6):701–708. https://doi.org/10.1007/s00128-015-1509-5
Abdel-Khalek AA (2016) Comparative evaluation of genotoxic effects induced by CuO bulk and nano-particles in Nile tilapia, Oreochromis niloticus. Water Air Soil Pollut 227:35. https://doi.org/10.1007/s11270-015-2737-3
Abdel-Khalek AA, Hamed A, Marie MA (2016) The accumulation potency of bulk and nano zinc metal and their impacts on the hematological and histological perturbations of Oreochromis niloticus. Water Air Soil Pollut 227:206. https://doi.org/10.1007/s11270-016-2908-x </uvertical-align:super;>
Abdel-Khalek AA, Elhaddad E, Mamdouh S, Marie MAS (2018) The chronic exposure to discharges of Sabal drain induces oxidative stress and histopathological alterations in Oreochromis niloticus. Bull Environ Contam Toxicol 101:92–98. https://doi.org/10.1007/s00128-018-2366-9
Abdel-Khalek AA, Hamed A, Hasheesh WS (2021) Does the adsorbent capacity of orange and banana peels toward silver nanoparticles improve the biochemical status of Oreochromis niloticus? Environ Sci Pollut Res. https://doi.org/10.1007/s11356-021-13145-9
Afifi M, Saddick S, Abu Zinada OA (2016) Toxicity of silver nanoparticles on the brain of Oreochromis niloticus and Tilapia zillii. Saudi J Biol Sci 23(6):754–760. https://doi.org/10.1016/j.sjbs.2016.06.008.
Ahmad T, Danish M (2018) Prospects of banana waste utilization in wastewater treatment: A review. J Environ Man 206:330–348. https://doi.org/10.1016/j.jenvman.2017.10.061
Aki T, Nara A, Uemura K (2012) Cytoplasmic vacuolization during exposure to drugs and other substances. Cell Biol Toxicol 28:125–131. https://doi.org/10.1007/s10565-012-9212-3
Akpomie KG, Conradie J (2020) Banana peel as a biosorbent for the decontamination of water pollutants. A review. Environ Chem Lett 18:1085–1112. https://doi.org/10.1007/s10311-020-00995-x
Alyasi H, Mackey HR, Loganathan K, McKay G (2020) Adsorbent minimisation in a two-stage batch adsorber for cadmium removal. J Ind Eng Chem 81:153–160. https://doi.org/10.1016/j.jiec.2019.09.003
Annadurai G, Juang RS, Lee DJ (2003) Adsorption of heavy metals from water using banana and orange peels. Water Sci Technol 47:185–190. https://doi.org/10.2166/wst.2003.0049
Arslan ÖC, Boyacioğlu M, Parlak H, Katalay S, Karaaslan MA (2015) Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey. Mar Pollut Bull 94:48–54. doi:https://doi.org/10.1016/j.marpolbul.2015.03.018
Asharani P, Hande MP, Valiyaveettil S (2009) Anti-proliferative activity of silver nanoparticles. BMC Cell Biol 10:65. https://doi.org/10.1186/1471-2121-10-65
Bacchetta C, Ale A, Simoniello MF, Gervasio S, Davico C, Rossi AS, Desimone MF, Poletta G, Lopez G, Monserrat JM, Cazenave J (2017) Genotoxicity and oxidative stress in fish after a short-term exposure to silver nanoparticles. Ecol Indicators 76:230–239. https://doi.org/10.1016/j.ecolind.2017.01.018
Bressan E, Ferroni L, Gardin C, Rigo C, Stocchero M, Vindigni V, Cairns W, Zavan B (2013) Silver nanoparticles and mitochondrial interaction. Int J Dent 31274:7. https://doi.org/10.1155/2013/312747
Costa S, Carvalho S, Costa C, Coelho P, Silva S, Santos LS, Gaspar JF, Porto B, Laffon B, Teixeira JP (2015) Increased levels of chromosomal aberrations and DNA damage in a group of workers exposed to formaldehyde. Mutagenesis 30(4):463–473. https://doi.org/10.1093/mutage/gev002
Fenech M, Kirsch-Volders M, Natarajan AT, Surralles J, Crott JW, Parry J, Norppa H, Eastmond DA, Tucker JD, Thomas P (2011) Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis 26(1):125–132. https://doi.org/10.1093/mutage/geq052
Fomina M, Gadd GM (2014) Biosorption: current perspectives on concept, definition and application. Bioresour Technol 160:3–14. https://doi.org/10.1016/j.biortech.2013.12.102
Genter MB, Newman NC, Shertzer HG, Ali SF, Bolon B (2012) Distribution and systemic effects of intranasally administered 25 nm silver nanoparticles in adult mice. Toxicol Pathol 40:1004–1013. https://doi.org/10.1177/0192623312444470
Hedayati SA, Farsani HG, Naserabad SS, Hoseinifar SH, Van Doan H (2019) Protective effect of dietary vitamin E on immunological and biochemical induction through silver nanoparticles (AgNPs) inclusion in diet and silver salt (AgNO3) exposure on Zebrafish (Danio rerio). Comp Biochem Physiol Part C Toxicol Pharmacol 222:100–107. https://doi.org/10.1016/j.cbpc.2019.04.004
Inshakova E, Inshakov O (2017) World market for nanomaterials: structure and trends. In: International conference on modern trends in manufacturing technologies and equipment (ICMTMTE). MATEC web of conference 129, pp 1–5. https://doi.org/10.1051/matecconf/201712902013
Kelly-Vargas K, Cerro-Lopez M, Reyna-Tellez S, Bandala ER, Sanchez-Salas JL (2012) Biosorption of heavy metals in polluted water, using different waste fruit cortex. Phys Chem Earth Parts (A/B/C) 37–39:26–29. https://doi.org/10.1016/j.pce.2011.03.006
Khan MS, Qureshi NA, Jabeen F (2017) Assessment of toxicity in freshwater fish Labeo rohita treated with silver nanoparticles. Appl Nanosci 7:167–179. https://doi.org/10.1007/s13204-017-0559-x
Liang S, Guo X, Feng N, Tian Q (2010) Isotherms, kinetics and thermodynamic studies of adsorption of Cu2+ from aqueous solutions by Mg2+/K+ type orange peel adsorbents. J Hazard Mater 174:756–762. https://doi.org/10.1016/j.jhazmat.2009.09.116
Massarsky A, Abraham R, Nguyen KC, Rippstein P, Tayabali AF, Trudeau VL, Moon TW (2014) Nanosilver cytotoxicity in rainbow trout (Oncorhynchus mykiss) erythrocytes and hepatocytes. Comp Biochem Physiol C 159:10–21. https://doi.org/10.1016/j.cbpc.2013.09.008
Maurer L, Meyer J (2016) A systematic review of evidence for silver nanoparticle-induced mitochondrial toxicity. Environ Sci Nano 3:311–322. https://doi.org/10.1039/C5EN00187K
Nabiev I, Mitchell S, Davies A, Williams Y, Kelleher D, Moore R, Gunko YK, Byrne S, Rakovish YP, Donegan JG, Sukhanova A, Conroy J, Cottell D, Gaponik N, Rogach A, Volkov Y (2007) Nonfunctionalized nanocrystals can exploit a cell’s active transport machinery delivering them to specific nuclear and cytoplasmic compartments. Nano Lett 7(11):3452–3461. https://doi.org/10.1021/nl0719832
Nnamdi AH, Briggs TMD, Togunde OO, Obanya HE (2019) Antagonistic effects of sublethal concentrations of certain mixtures of metal oxide nanoparticles and the bulk (Al2O3, CuO, and SiO2) on gill histology in Clarias gariepinus. J Nanotechnol 2019:1–11. https://doi.org/10.1155/2019/7686597
Oyewo OA, Onyango MS, Wolkersdorfer C (2018) Lanthanides removal from mine water using banana peels nanosorbent. Int J Environ Sci Technol 15:1265–1274. https://doi.org/10.1007/s13762-017-1494-9
Pathak PD, Mandavgane SA, Kulkarni BD (2017) Fruit peel waste: characterization and its potential uses. Curr Sci 113(3):444–454. https://doi.org/10.18520/CS/V113/I03/444-454
Recio L, Hobbs C, Caspary W, Witt KL (2010) Dose-response assessment of four genotoxic chemicals in a combined mouse and rat micronucleus (MN) and Comet assay protocol. J Toxicol Sci 35(2):149–162. https://doi.org/10.2131/jts.35.149
Simoniello MF, Gigena F, Poletta G, Loteste A, Kleinsorge E, Campana M, Scagnetti J, Parma MJ (2009) Alkaline comet assay for genotoxic effect detection in neotropical fish Prochilodus lineatus (Pisces, Curimatidae). Bull Environ Contam Toxicol 83(2):155–158. https://doi.org/10.1007/s00128-009-9771-z
Singh NB, Nagpal G, Agrawal S, Rachna (2018) Water purification by using adsorbents: a review. Environ Technol Innov 11:187–240. https://doi.org/10.1016/j.eti.2018.05.006
Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35(3):206–221. https://doi.org/10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
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This study was supported by the Researchers Supporting Project Number (RSP-2021/25), King Saud University, Riyadh, Saudi Arabia.
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All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by AAA-K, SA-Q, and RA-G. The first draft of the manuscript was written by AAA-K and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Abdel-Khalek, A.A., Al-Quraishy, S. & Abdel-Gaber, R. Silver Nanoparticles Induce Time- and Tissue-Specific Genotoxicity in Oreochromis niloticus: Utilizing the Adsorptive Capacities of Fruit Peels to Minimize Genotoxicity. Bull Environ Contam Toxicol 108, 300–308 (2022). https://doi.org/10.1007/s00128-021-03342-0
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DOI: https://doi.org/10.1007/s00128-021-03342-0